Naslov
Escherichia coli metabolic network modelling

Autori
Pleša, Ivana ; Kurtanjek, Želimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
PrimMath[2001] / Ungar, Š. - Zagreb : Prirodoslovno-matematički fakultet Sveučilišta u Zagrebu, 2001

Skup
PrimMath[2001]

Mjesto i datum
Zagreb, Hrvatska, 27.09.2001. - 28.09.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Escherichia coli; metabolic engineering; computer simulation

Sažetak
This work presents mathematical model of Escherichia coli metabolism based on kinetic data and annotated genome information by J.S. Edwards(1). Published reaction rates and kinetic parameters are used for derivation of mass balances for the key metabolic networks. The balances are given in dynamic form, which enables simulation of transient metabolic behaviour under varying experimental conditions. Applied is stoichiometric analysis of reaction rate constraints. The computer model is based on a set of stiff nonlinear ordinary differential equations (ODE) with specified initial conditions. Applied is NDSolve numerical integration program for evaluation of the model equations. The results are presented in graphical form enabling visualisation of key metabolic pathways. The results are discussed with various published data from literature

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA