Naslov
Aspergillus screening difficulties in pulmonary cytology samples

Autori
Harabajsa, Suzana ; Markanović, Manda ; Pešut, Ana ; Vrabec Branica, Božica ; Šimić, Vesna ; Mareković, Ivana ; Smojver-Ježek, Silvana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, pp prezentacija, stručni

Skup
Toraks 2019

Mjesto i datum
Zagreb, Hrvatska, 10.04.2019. - 13.04.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
Aspergillus ; cytology ; microbiology ; fungal culture

Sažetak
Aim: To present Aspergillus screening difficulties in pulmonary cytology samples in correlation with microbiological fungal culture results. Methods: Aspergillus is the most common fungal organism followed by Candida in pulmonary cytology samples. The six year retrospective analysis included 27 cytology samples and corresponding cytological final reports of Aspergillus in Pulmonary Cytology Division. Microscopical findings in May Grünwald Giemsa slides (MGG) were uniform, septate, dichotomous, 45° branching (Y-shaped) hyphae consistent with Aspergillus. The cytological final reports were correlated with the microbiological fungal culture results. Results: Among 27 cytology samples with Aspergillus resembling morphology were 14 washing aspirates, six bronchial brushings, four imprint materials, two sputum samples and one transbronchial fine needle aspiration. Inflammation elements, poorly preserved hyphae and necrosis were also present in cytology samples. Cytology samples and microbiological fungal cultures were simultaneously obtained in 16 (16/27) cases. Fungal culture reports were positive for A. fumigatus, Aspergillus spp, A. flavus or A. niger in 14 (14/16) and negative in two (2/16) cases. In three (3/14) cases of Aspergillus positive fungal cultures and two (2/2) cases of Aspergillus negative fungal cultures Candida was also present. In six (6/27) cases with positive cytology samples subsequent fungal cultures were obtained. All six subsequent fungal cultures were negative for Aspergillus but one (1/6) of them had a prior history report of Aspergillus positive fungal culture and two (2/6) were positive for Candida albicans. In five cases cytological positive final reports were correlated with previous microbiological fungal culture results. Among previous fungal cultures, one (1/5) was positive for Aspergillus, one (1/5) for Candida albicans and three (3/5) were both negative. Conclusion: Morphological identification of Aspergillus in pulmonary cytology samples can be challenging. There is no morphological difference in cytological samples to differentiate between colonization and invasive infection. To determine the significance of cytological final reports of Aspergillus, microbiological fungal culture, fungal biomarkers, clinical and radiographic findings should be considered.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA