Naslov
Structured modelling of microorganism metabolic network

Autori
Kurtanjek, Želimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
PrimMath[2001] / Ungar, Š. - Zagreb : Prirodoslovno-matematički fakultet Sveučilišta u Zagrebu, 2001

Skup
PrimMath[2001]

Mjesto i datum
Zagreb, Hrvatska, 27.09.2002. - 28.09.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
structured modelling; metabolic engineering; computer simulation

Sažetak
This work presents the structured model of Saccharomyces cerevisiae metabolism based on kinetic data published by Steinmeyer and Shuler(1). The model is expressed as a set of stiff ODE equations with initial conditions. The equations correspond to mass balances of principal reactions corresponding to key metabolic pathways. The kinetic expressions are of Michaelis-Menten structure with inclusion of various modes of feedfarward and feedback activation and inhibition. The model accounts for a yeast cell assimilation of extracellular carbon, nitrogen, oxygen and amino acids. The balances are given in dynamic form, which enables simulation of transient metabolic behaviour under varying experimental conditions. Energy status of a yeast cell is expressed by pseudo energy charge model. Intracellular reactions are The computer model is based on a set of stiff nonlinear ordinary differential equations (ODE) with specified initial conditions. Applied is NDSolve numerical integration program for evaluation of the model equations and SingularValues algorithm for analysis of stoichiometric constraints imposed to intracellular reactions(2). The model is aimed to analysis of metabolism control.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA