Naslov
The effect of hyperbaric oxygenation on Na+, K+-ATPase activity in optic nerve of ischemic rats

Autori
Mršić Pelčić, Jasenka ; Pelčić, Goran ; Vitezić, Dinko ; Župan, Gordana ; Simonić, Ante

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Periodicum biologorum (ISSN 0031-5362), Vol 103, Supp 1 / - Zagreb : Hrvatsko prirodoslovno društvo, 2001

Skup
3rd Croatian Congress of Pharmacology with International Participation

Mjesto i datum
Zagreb, Hrvatska, 18.09.2001. - 21.09.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Brain ischemia; Hyperbaric oxygenation; Na+; K+-ATPase activity; Optic nerve; Rats

Sažetak
Introduction: The aim of our study was to examine the influence of hyperbaric oxygenation (HBO) on Na+, K+-ATPase activity in optic nerve of ischemic rats. Materials and Methods: Animals: Hannover-Wistar rats, weighing 250 g. Models: Global cerebral ischemia: vertebral arteries were occluded by electrocautery at the level of the first cervical vertebra.The day after, the common carotid arteries were exposed and a forebrain ischemia was produced by tightening the carotid artery clips for 20 min. Na+, K+-ATPase assay: Na+, K+-ATPase activity was determined spectrophotometrically by modification of the method described by Stefanovic et al (1974). Hyperbaric oxygenation: Animals have been exposed to HBO during seven consecutive days, at 222.86 kPa pressure of 99.5% oxygen, lasting 60 min. Procedure: Animals were exposed to global cerebral ischemia and after different reperfusion period (immediately after ischemia, 30 min, 1, 2, 24, 48, 72 and 168 hrs), Na+, K+ -ATPase activity was measured. A part of ischemic animals were exposed to HBO after reperfusion. Results: We found significant decrease in Na+, K+-ATPase activity in periods from 30 min to 7 days after ischemia. The most profound decrease was registered in 24 hrs reperfusion time. Treatment with HBO prevented enzymatic inhibition in animals exposed to hyperbaric oxygen in a period up to 24 hrs after ischemic injury. Conclusions: Decrease in Na+, K+-ATPase activity was evident in ischemic animals exposed to different reperfusion intervals. Treatment with HBO maintained enzymatic activity up to control levels, and exerted neuroprotective effect.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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