Naslov
Diagnostic Accuracy Of Siemens Advia 2120i Hematology Analyzer İn Detecting Pseudothrombocytopenia

Autori
Bogić, Anamarija ; Vuljanić, Dora ; Petro, Dubravka ; Radišić Biljak, Vanja ; Šimundić, Ana-Maria

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Skup
5th EFLM-UEMS European Joint Congress in Laboratory Medicine

Mjesto i datum
Antalya, Turska, 10.10.2018. - 13.10.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
hematology analyser, pseudothrombocytopenia, flagging efficiency

Sažetak
Aim: The aim of the study was to investigate the diagnostic accuracy of Siemens Advia 2120i hematology analyzer in detection of pseudothrombocytopenia. Materials and methods: Total of 160 K2EDTA whole blood samples (platelet count <100x109/L) were included. All samples were inspected for presence of platelet clumps in peripheral blood smears (stained with May-Grünwald Giemsa) with manual microscopy using Motic BA410, as a reference method. The presence of the two categories of flags (platelet clumps and large platelets) were recorded. Specificity and sensitivity for each flag category, as well as for their combination, were calculated. Criteria for sensitivity and specificity (2016 state-of-the-art by Vis and Huisman) were >80% and >98%, respectively. Results: Platelet clumps were confirmed in 10/160 and large platelets in 9/160 samples. Specificity of Advia 2120i in detecting platelet clumps was excellent (>99%), while sensitivity was poor (36%). The sensitivity and specificity of Advia 2120i in detecting large platelets were satisfactory (82% and 88%, respectively). Combining both flags as an alert for peripheral blood smear review, improved the diagnostic accuracy of detecting platelet clumps in patients samples (specificity 99%, sensitivity 83%) as some of the truly positive samples had overlaps between clumps and large platelets. Conclusions: Siemens Advia 2120i hematology analyzer has acceptable accuracy in detection of pseudothrombocytopenia, with a false negative rate of 16.7% (2/12 samples). Based on these results, our laboratory protocol is to perform microscopic examination of all samples with platelets count <100x109/L if either of the morphology flags (platelet clumps and large platelets) is reported by the analyser.

Izvorni jezik
Engleski

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