Naslov
Izrada i primjena DNA mikročipa za određivanje ekspresije gena kod kavezne atlantske plavoperajne tune (Thunnus thynnus L., 1758)
(Development and application of a DNA microarray for gene expression profiling in captive Atlantic bluefin tuna (Thunnus thynnus L., 1758))

Autori
Trumbić, Željka

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, doktorska disertacija

Fakultet
Sveučilište u Osijeku, Institut Ruđer Bošković, Sveučilište u Dubrovniku

Mjesto
Osijek

Datum
15.09

Godina
2015

Stranica
115

Mentor
Mladineo, Ivona ; Taggart, John

Ključne riječi
Thunnus thynnus ; Didymosulcus katsuwonicola ; sekvenciranje transkriptoma ; DNA mikročip ; Trematoda ; ultrastruktura
(Thunnus thynnus ; Didymosulcus katsuwonicola ; transcriptome sequencing ; DNA microarray ; Trematoda ; ultrastructure)

Sažetak
Atlantic bluefin tuna Thunnus thynnus is migratory fish with distinct physiology and high economic value. In this study mixed-tissue normalized cDNA library was constructed, pyrosequenced, assembled and annotated. It was used as a template for construction of T. thynnus-specific DNA microarray with 15 000 probes used to infer tissue-specific gene expression profiles. The relationship between bluefin as a host and digenean trematode Didymosulcus katsuwonicola was further investigated employing DNA microarray and transmission electron microscopy.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina, Biotehnologija

POVEZANOST RADA