Naslov
CENH3 proteins as epigenetic determinants of Tribolium centromeres

Autori
Gržan, Tena ; Meštrović, Nevenka ; Plohl, Miroslav ; Mravinac, Brankica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Molecular Biophysics: ABC of the puzzle of Life. The third COST-sponsored ARBRE-MOBIEU / - Zagreb : Hrvatsko biofizičko društvo, 2019, 91-91

ISBN
978-953-7941-28-4

Skup
3rd COST-sponsored ARBRE-MOBIEU plenary meeting Molecular Biophysics - ABC of the puzzle of Life

Mjesto i datum
Zagreb, Hrvatska, 18.03.2019. - 20.03.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
CENH3 ; centromere ; Tribolium castaneum ; Tribolium freemani

Sažetak
The flour beetles Tribolium castaneum and Tribolium freemani, the important world-wide pests of stored agricultural products, are able to mate, but produce sterile hybrid progeny in reciprocal crosses. In the context of genetic diversity between closely related species, we focused our research on DNA and protein aspects of their centromere regions as crucial chromosomal structures. By using the histone H3 sequence as query to search genome databases, in both species we first identified the centromeric histone H3 variants (CENH3), epigenetic hallmarks of active centromeres. T. castaneum cCENH3 and T. freemani fCENH3 H3-like variants share 80% identity in amino acid sequences. Their species-specific N-terminal tails were used to raise antibodies that localized cCENH3 and fCENH3 proteins exclusively to centromeres of T. castaneum and T. freemani, respectively. We further performed ChIP-Seq experiments, which predominantly associated cCENH3 and fCENH3 nucleosomes with a major satellite DNA in each species. As supported by IF-FISH analyses, highly abundant satellite DNAs, TCAST in T. castaneum and TFREE in T. freemani, spread both at pericentromeric and functional centromeric regions of all chromosomes. TCAST and TFREE satellite DNAs account for large fractions (>20%) of their indigenous genomes, and they differ dramatically in monomer unit length (360 bp versus 166 bp) as well as in nucleotide sequence, thus being fundamentally species- specific. In terms of the reproductive isolation between T. castaneum and T. freemani, these findings argue in favor of adaptive evolution of cCENH3 and fCENH3 proteins that might be driven by a response to changes in satellite DNA sequences at centromere loci.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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