Naslov
Istraživanje hibridnih struktura G-kvadrupleksa spektroskopijom površinski pojačanog Ramanova raspršenja
(Study of hybrid G-quadruplex structures by surface-enhanced Raman scattering spectroscopy)

Autori
Kenđel, Adriana ; Papi, Francesco ; Ratkaj, Marina ; Piantanida, Ivo ; Miljanić, Snežana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
26. hrvatski skup kemičara i kemijskih inženjera s međunarodnim sudjelovanjem, 4. simpozij Vladimir Prelog 9.–12. travnja 2019. : knjiga sažetaka / Galić, Nives ; Rogošić, Marko - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2019, 56-56

ISBN
978-953-6894-67-3

Skup
26. hrvatski skup kemičara i kemijskih inženjera (26HSKIKI) ; 4. simpozij Vladimir Prelog

Mjesto i datum
Šibenik, Hrvatska, 09.04.2019. - 12.04.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
G-quadruplex ; surface-enhanced Raman spectroscopy

Sažetak
G-quadruplexes (G4) are nucleic acid structures formed by guanine-rich sequences, involved in multiple processes in living cells, including DNA transcription, replication, translation and end-protection of telomeres [1]. Human telomeric sequences can fold into three main G4 arrangements, namely parallel, antiparallel and hybrid, whereby parallel and hybrid structures are the most interesting since they are usually found in solutions rich in potassium that are quite similar to the conditions within the living cells [2, 3]. Surface-enhanced Raman scattering (SERS) and circular dichroism (CD) spectroscopy were used to investigate the human telomeric sequences Tel26 d[A3G3(TTAGGG)3A2] and wtTel26 d[(TTAGGG)4T2] at different concentrations (10, 30, 50 µM) in potassium solutions. Studied telomeric sequences can fold into hybrid-1 and hybrid-2 G4 forms in dilute solutions, differing in the order of the loops arrangement [4]. In the SERS spectra characteristic G-quadruplex marker bands were noted. Intensity ratios of guanine characteristic SERS bands revealed C2’-endo/syn and C2’-endo/anti conformations in G-quadruplexes, while intensity ratios of adenine and guanine breathing bands proved presence of the folded telomeric sequences on the sliver surface [5]. Though the SERS spectra of Tel26 and wtTel26 shown similar patterns, as expected because of hybrid-1 and hybrid-2 structural resemblance, a series of different spectral features were observed, indicating that adenine and thymine residues play a determinant role in interactions with the metallic surface. It was suggested that Tel26 tends to adsorb by its double-chain-reversal side loop, while wtTel26 most likely approaches the metallic surface by one of its lateral loops. CD spectroscopy confirmed the folding of Tel26 and wtTel26 into the hybrid G4 structures, which were not affected by adsorption onto the enhancing silver nanoparticles.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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