Naslov
Extraction of rutin and rosmarinic acid from Satureja montana L. with deep eutectic solvents

Autori
Jakovljević, Martina ; Vladić, Jelena ; Molnar, Maja ; Jokić, Stela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
26. HSKIKI Book of Abstracts / Galić, Nives ; Rogošić, Marko - Zagreb, 2019, 123-123

Skup
26. hrvatski skup kemičara i kemijskih inženjera (26HSKIKI) ; 4. simpozij Vladimir Prelog

Mjesto i datum
Šibenik, Hrvatska, 09.04.2019. - 12.04.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Satureja montana, extraction, deep eutectic solvents, rutin, rosmarinic acid

Sažetak
Given the growing use of bioactive components in the industry, the development of new and green methods for their extraction is crucial. Solvents play an important role in chemical process of mass and heat transfer so selecting suitable solvents is one of the most important steps in the extraction process. Deep eutectic solvents (DESs), which are composed of a hydrogen bond acceptor (HBA) (usually choline chloride) and hydrogen bond donor (HBD) components, stand out as a promising substitution to conventional solvents due to their great properties as non‐toxicity, thermal stability, easy preparation, biodegradability and low price of components. Satureja montana is an aromatic and medicinal plant used in traditional medicine due to composition which include antioxidant components, as well as in cooking as a spice and in the cosmetics industry [1, 2]. In this study, a simple extraction method based on DES was used for the extraction of rutin and rosmarinic acid from Satureja montana L. Five different choline chloride based deep eutectic solvents (urea, sorbitol, butan‐1, 4 diol, lactic and levulinic acid as HBD) were tested and compared with water as solvent. Besides, the influence of amount of water added (10%, 30%, 50%) and temperatures (30, 50, 70 °C) on the rosmarinic acid and rutin content in extracts was investigated. After extraction, the components were detected and quantified using high performance liquid with diode‐array detector (HPLC‐DAD. Among tested DES, choline chloride: lactic acid (1:2) and choline chloride: levulinic acid (1:2) were the most suitable for extraction of rutin, while in the case of rosmarinic acid similar amount was obtained with all of tested solvents. Depending on the parameters and the solvents used, the quantities of extracted rutin ranged from 1.40 to 17.29 μg mg–1 of plants, while the quantities of extracted rosmarinic acid ranged from 0.21 to 7.84 μg mg–1 of plants. On the other hand, by extraction with water at different temperatures, 5.16 – 6.27 mg–1 of rutin and 2.30 – 3.81 μg mg–1 of rosmarinic acid was extracted. The obtained results indicated that deep eutectic solvents could be an alternative to traditional solvents for extraction of rutin and rosmarinic acid from Satureja montana.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA