Naslov
Reduction in nuber of hippocampal calretinin- and parvalbumin-immunoreactive interneurons in cuprizone-induced demyelination in mice

Autori
Fenrich, Matija ; Zjalić, Milorad ; Blažetić, Senka ; Heffer, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
OSCON Abstract Book / - Osijek : Faculty of Medicine Osijek, 2019, 66-66

Skup
1st International Translational Medicine Congress of Students and Young Physicians (OSCON 2019)

Mjesto i datum
Osijek, Hrvatska, 07.02.2019. - 08.02.2019

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Interneurons ; Hippocampus ; Parvalbumins ; Calbindin 2

Sažetak
Introduction: Inhibitory interneurons play prominent roles in the function of the cerebral cortex. They are involved in modulation and plasticity of the cortical micro-columns. Changes in phenotype of interneurons have been observed in schizophrenia, seizures, autism and many other neurodevelopmental disorders. Our hypothesis is that disturbance of plasticity through demyelination results in a change of phenotype of hippocampal interneurons. Materials and methods: The study was conducted on six C57BL/6 mice, 3 of which were fed on chow containing 0.2% cuprizone in order to induce demyelination, and 3 were fed on standard chow. The mice were anesthetized with isoflurane, and perfused with 4% paraformaldehyde in phosphate buffer saline (pH = 7.4). Brains were isolated, perfused, cryoprotected using 30% saccharose, and stored at -80 °C. The specimens were cut on cryostat (Leica CM3050S) in 35 microns thick slices, and immunohistochemistry for parvalbumin (PV) and calretinin (CR) was conducted. The following hippocampal regions of interest were identified: dentate gyrus (DG), cornu Ammonis 1 (CA1) and cornu Ammonis 3 (CA3). Slices were photographed on Zeiss Axioskop 2 MOT microscope, and an intensity threshold was applied on each micrograph based on the intensity measured on negative controls. A number of neuronal somata per area unit was counted in respective hippocampal regions in both hemispheres using ImageJ (version 1.52i). The extent of demyelination was determined in a previous study. Results: A statistically significant reduction in number of CR-interneuronal somata per µm² was observed in DG of animals fed on cuprizone (p = 0.009). The count of PV-interneuronal somata per µm² was also lesser in mice fed on cuprizone compared to the control, in DG (p = 0.015) and in CA1 (p = 0.02). Conclusion: Our results suggest that a reduction in number of CR- and PV-immunoreactive interneurons takes place in hippocampi in the cuprizone model of demyelination. However, more interneuronal markers are to be included in future studies for more elusive conclusions.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA