Naslov
Plant regeneration and clonal fidelity assessment of subendemic species Iris illyrica Tomm. originated from Croatia

Autori
Bošnjak Mihovilović, Anita ; Barić, Marijana ; Bolarić, Snježana ; Safner, Toni ; Ćurković-Perica, Mirna ; Mitić, Božena ; Bohanec, Borut ; Habuš Jerčić, Ivanka ; Kereša, Snježana

Izvornik
Journal of central European agriculture (1332-9049) 20 (2019), 1; 415-430

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
auxins, explant browning, flow cytometry, Iris, leaf base explants,

Sažetak
The present study was undertaken to evaluate efficiency of callogenesis and regeneration by somatic embryogenesis of the subendemic Iris species Iris illyrica from Croatia and to select highly regenerative donor plants/genotypes. Leaf base segments were used as explants. Callogenesis and somatic embryogenesis were induced on MS media supplemented with: (1) 4.52 uM 2, 4-dichlorophenoxyacetic acid (2, 4-D) + 4.83 uM 1-naphthaleneacetic acid (NAA) + 0.46 uM kinetin Kin) ; (2) 4.52 uM 2, 4-D + 4.6 uM Kin ; (3) 13.4 uM NAA + 2.3 uM Kin. Transfer of embryogenic calli onto hormone-free medium enabled the development of mature somatic embryos. Frequency of callogenesis was influenced by the donor plant. Among 15 donor plants tested, 3 of them exhibited high regeneration capability and produced 87 regenerants. Seven morphological traits were observed in order to assess phenotypic variability of flowering regenerants. Regenerants with higher values of fall and standard width and length show potential for further breeding of new varieties of Illyrian iris. Flowering and non-flowering renerants had the same ploidy level as donor plants. Also, the nuclear DNA content (2C = 12.936 ± 0.038 pg) of this species was estimated for the first time using flow cytometry.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija)

POVEZANOST RADA