Naslov
Peptide Mapping for the Characterization of Proteins

Autori
Josic, Djuro ; Schulz, Petra ; Biesert, Lothar ; Hoffer, Lutz ; Schwinn, Horst ; Kordis-Krapez, Mira ; Strancar, Ales

Izvornik
Journal of chromatography. B, Biomedical sciences and applications (1387-2273) 694 (1997), 2; 253-269

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Peptides ; Peptide Mapping ; Proteins

Sažetak
This text is devoted to the characterization of recombinant DNA-derived proteins by peptide mapping. It describes new technological procedures including capillary electrophoresis, analysis of glycopeptides and the use of electrospray and matrix-assisted laser desorption mass spectrometry. The book presents practical procedures for preparing a protein sample, the enzyme digestion, choice of separation method and procedures for the structural analysis of the separated species. Many figures of peptide maps illustrate typical results. Tables of summary information about digestion, separation conditions, and analyses of important protein samples are also presented.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA