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Pregled bibliografske jedinice broj: 990310

Identification of members of the annexin family in the detergent-insoluble fraction of rat Morris hepatoma plasma membranes

Clifton, James G.; Brown, Mari Kino; Huang, Feilei; Li, Xuesong; Reutter, Werner; Hofmann, Werner; Hixson, Douglas C.; Josic, Djuro
Identification of members of the annexin family in the detergent-insoluble fraction of rat Morris hepatoma plasma membranes // Journal of Chromatography A, 1123 (2006), 2; 205-211 doi:10.1016/j.chroma.2006.02.020 (međunarodna recenzija, članak, znanstveni)

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Naslov
Identification of members of the annexin family in the detergent-insoluble fraction of rat Morris hepatoma plasma membranes

Autori
Clifton, James G. ; Brown, Mari Kino ; Huang, Feilei ; Li, Xuesong ; Reutter, Werner ; Hofmann, Werner ; Hixson, Douglas C. ; Josic, Djuro

Izvornik
Journal of Chromatography A (0021-9673) 1123 (2006), 2; 205-211

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Morris hepatoma 7777 plasma membrane ; Selective extraction ; Annexins ; Cancer biomarkers ; Mass spectrometry

Sažetak
For proteomic analysis, plasma membranes of rat hepatocellular carcinoma Morris hepatoma 7777 were selectively solubilized according to the previously developed method [D. Josic, K. Zeilinger, Methods Enzymol. 271 (1996) 113–134]. If the Triton X100 insoluble pellet is subsequently extracted, several proteins can be solubilized. These proteins can be classified in two groups according to their molecular size. The proteins with apparent molecular weights in SDS-PAGE between 70 and 75 kDa belong to the first group. Smaller proteins, with apparent molecular weights between 30 and 45 kDa, are members of the second group. The main protein of higher molecular weight was also found in the Triton X100 insoluble extract from normal rat liver plasma membranes. This protein was identified as Annexin A6. The proteins from the second group are practically absent in the Triton X100 insoluble extract from rat liver. These proteins are present in relatively high concentrations in plasma membranes of Morris hepatoma 7777. Both groups of detergent-insoluble proteins from Morris hepatoma 7777 were further analyzed with SELDI-TOF and LC electrospray ionization mass spectrometry. From the first group, Annexin A6, together with two other integral plasma membrane proteins, was identified. In the second group of proteins with apparent molecular weights between 30 and 45 kDa, further members of the annexin family, Annexins A1, A2, A4, A5 and A7 were identified. The possible role of these low molecular size annexins as potential cancer biomarkers is discussed.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA

Ustanove:
Sveučilište u Rijeci - Odjel za biotehnologiju

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Clifton, James G.; Brown, Mari Kino; Huang, Feilei; Li, Xuesong; Reutter, Werner; Hofmann, Werner; Hixson, Douglas C.; Josic, Djuro
Identification of members of the annexin family in the detergent-insoluble fraction of rat Morris hepatoma plasma membranes // Journal of Chromatography A, 1123 (2006), 2; 205-211 doi:10.1016/j.chroma.2006.02.020 (međunarodna recenzija, članak, znanstveni)
Clifton, J., Brown, M., Huang, F., Li, X., Reutter, W., Hofmann, W., Hixson, D. & Josic, D. (2006) Identification of members of the annexin family in the detergent-insoluble fraction of rat Morris hepatoma plasma membranes. Journal of Chromatography A, 1123 (2), 205-211 doi:10.1016/j.chroma.2006.02.020.
@article{article, author = {Clifton, James G. and Brown, Mari Kino and Huang, Feilei and Li, Xuesong and Reutter, Werner and Hofmann, Werner and Hixson, Douglas C. and Josic, Djuro}, year = {2006}, pages = {205-211}, DOI = {10.1016/j.chroma.2006.02.020}, keywords = {Morris hepatoma 7777 plasma membrane, Selective extraction, Annexins, Cancer biomarkers, Mass spectrometry}, journal = {Journal of Chromatography A}, doi = {10.1016/j.chroma.2006.02.020}, volume = {1123}, number = {2}, issn = {0021-9673}, title = {Identification of members of the annexin family in the detergent-insoluble fraction of rat Morris hepatoma plasma membranes}, keyword = {Morris hepatoma 7777 plasma membrane, Selective extraction, Annexins, Cancer biomarkers, Mass spectrometry} }
@article{article, author = {Clifton, James G. and Brown, Mari Kino and Huang, Feilei and Li, Xuesong and Reutter, Werner and Hofmann, Werner and Hixson, Douglas C. and Josic, Djuro}, year = {2006}, pages = {205-211}, DOI = {10.1016/j.chroma.2006.02.020}, keywords = {Morris hepatoma 7777 plasma membrane, Selective extraction, Annexins, Cancer biomarkers, Mass spectrometry}, journal = {Journal of Chromatography A}, doi = {10.1016/j.chroma.2006.02.020}, volume = {1123}, number = {2}, issn = {0021-9673}, title = {Identification of members of the annexin family in the detergent-insoluble fraction of rat Morris hepatoma plasma membranes}, keyword = {Morris hepatoma 7777 plasma membrane, Selective extraction, Annexins, Cancer biomarkers, Mass spectrometry} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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