Naslov
Stability of faecal calprotectin extracts

Autori
Vrtarić, Alen ; Božović, Marija ; Nikolac Gabaj, Nora ; Culej, Jelena ; Milevoj Kopčinović, Lara ; Herceg, Ivona

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Clinical Chemistry and Laboratory Medicine / Plebani, Mario - Berlin : Walter de Gruyter, 2019, EA42-eA42

Skup
5th EFLM Conference on Preanalytical Phase

Mjesto i datum
Zagreb, Hrvatska, 22.03.2019. - 23.03.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
faecal calprotectin, extraction, stool

Sažetak
BACKGROUND-AIM: The BÜHLMANN CALEX® Cap (BÜHLMANN Laboratories AG, Switzerland) is a single use tube filled with extraction buffer used for extraction of calprotectin (fCal) from human stool samples. According to manufacturer’s specifications, fCal extract is stable 6 days at 2-8 °C. The aim of this study was to verify whether the manufacturer’s criteria are met and test if fCal extracts are stable if stored for a longer period (i.e. for 10 days at 2-8 °C). METHODS: Faecal calprotectin was extracted from N = 10 stool samples according to the manufacturer’s specified protocol. Extracts were then aliquoted in 6 aliquots ; initial fCal was measured on the day of extraction, while the remaining aliquots were stored at 2-8 °C for 1, 3, 5, 7 and 10 days, respectively. Measurements were made on the Abbott Architect c8000 (Abbott Laboratories, Abbott Park, USA) using BÜHLMANN fCAL® turbo reagent (BÜHLMANN Laboratories AG, Switzerland). Bias from the initial fCal concentration was calculated for each individual sample. Mean bias was calculated for each storage period and compared to Instand criteria which is 36%. Statistical analysis was performed using Medcalc (Ostend, Belgium) Friedman ANOVA test. P-value was set at < 0.05. RESULTS: The initial fCal concentration expressed as median (interquartile range) was 198.8 (58.7-731.9) mg/kg. Median concentrations measured in extracts stored for 1, 3, 5, 7 and 10 days were 213.5 (62.4-716.3), 204.5 (54.4-690.3), 207.0 (51.2-682.4), 205.0 (53.7-679.5) and 189.3 (56.2-657.6) mg/kg, respectively. Bias from the initial fCal concentration for extracts stored for 1, 3, 5, 7 and 10 days were -3.52 ; -4.41 ; -0.26 ; -7.03 and -3.41%, respectively. No difference between the initial fCal and fCal measured in extracts stored in the observed time periods was found (P = 0.830). CONCLUSIONS: Faecal calprotectin extract stability met the criteria specified by the manufacturer. We confirmed that fCal extracts might be stored for periods up to 10 days at 2- 8 °C.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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