Naslov
Analysis of aggregates of human immunoglobulin G using size-exclusion chromatography, static and dynamic light scattering

Autori
Ahrer, K. ; Buchacher, A. ; Iberer, G. ; Josic, D. ; Jungbauer, A.

Izvornik
Journal of Chromatography A (0021-9673) 1009 (2003), 1-2; 89-96

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
immunoglobulin G ; size-exclusion chromatography ; static light scattering ; dynamic light scattering

Sažetak
Large aggregates (Mr: 10(6)-10(7) g/mol) of human immunoglobulins are present in extremely small concentrations in IgG preparations (<0.1%). Traces of large protein aggregates cannot be determined by conventional size-exclusion chromatography (SEC) using UV detection due to limitations in sensitivity. The conventional analysis of IgG by SEC is limited to dimers and oligomers. Using light scattering it is possible to determine significant differences concerning the aggregate composition and the extent of protein aggregation in samples of different process steps. Two different pilot preparations were analyzed by SEC with UV and static light scattering detection and compared to dynamic light scattering in the batch mode. The change of large aggregates could be monitored and data were corroborated by dynamic light scattering.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA