Naslov
Ascendant of lactic acid bacteria Lactobacillus brevis L62 over Salmonella enterica serotype Typhimurium StrR in mixed culture

Autori
Bačun-Družina, Višnja ; Huđek, Ana ; Butorac, Ana ; Durgo, Ksenija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the 6th Congress of the Microbiologists of Macedonia with international participation / Panovski, Nikola - : Macedonian Medical Association and Macedonian Microbiological Society, 2018, 230-231

Skup
6th Congress of the Microbiologists of Macedonia with international participation and Federation of European Microbiological Societies supported Symposium Emerging Infections (FEMS 2018)

Mjesto i datum
Ohrid, Sjeverna Makedonija, 30.05.2018. - 02.06.2018

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
lactic acid bacteria, L. plantarum, S. enterica, GASP phenotype

Sažetak
INTRODUCTION: Almost every environments niche harbours multi-species community with mixed bacterial cultures which coexist in different relationships. In laboratory conditions, bacterial interaction can be followed by cultivation mixed bacterial cultures under the hunger conditions during prolonged stationary phase with bacterial species of different ages to distinguish their competitive potential. To compared and measure competitive growth in mixed bacterial cultures have great importance for microbial food safety. OBJECTIVES: To compare the competitive growth of one and six days old S. enterica serotype Typhimurium LT21 bacterial cultures and one-day old Lactobacillus brevis L62 bacterial culture were mixed in ratios 1:1 and 1:10 (vol:vol) and cultivated at 37 ºC. Considering the described phenomenon of constant protein synthesis in bacterial culture during stationary phase, the qualitative protein profile was measured. MATERIAL: The bacterial strains used in this paper were: S. enterica serotype Typhimurium LT21 wild type and strains harbouring the resistance to streptomycin (StrR), and Lactobacillus brevis L62 harbouring the resistance to nalidixic acid (NalR). METHODS: In a typical growth advantage in stationary phase competition experiment, cells from a 6-day-old culture are inoculated as a numerical minority (1:10 vol/vol) into a young (1-day-old) culture or in ratios 1:1 (vol:vol). Proteomic analysis of cells was performed by purification and separation of the peptides by liquid chromatography. Mass spectra were analyzed on a MALDI TOF/TOF mass spectrometer (Autoflex Speed, Bruker Daltonics, Bremen, Germany). RESULTS: Lactobacillus brevis L62 (1-day-old) cell cultures grown in a mixed culture with 1-day-old bacterial culture of Salmonella enterica serotype Typhimurium LT21 in ratio 1:1 or 10: 1 at 37 ºC on a minimal substrate showed strong competitive growth. S. enterica serotype Typhimurium LT21 (6-days-old) cell cultures grown in a mixed culture with 1-day-old cells of L. brevis L62 in ratio 1:10 at 37 ºC on a minimal basis show abortive phenotype of growth advantage in stationary phase. Proteomic analysis showed that proteins required for essential cellular processes are present in L. brevis L62 cells. CONCLUSION: Bacterial cells of L. brevis L62 have shown a strong competitive growth against 1-day and 6-day-old cells of S. enterica serotype Typhimurium and can be used to protect and remove contamination with antibiotic resistance pathogenic species of Enterobacteria. On this way can be prevent and control human foodborne pathogen.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA