Pregled bibliografske jedinice broj: 985423
Non-fluorescent mutant of green fluorescent protein sheds light on the mechanism of chromophore formation
Non-fluorescent mutant of green fluorescent protein sheds light on the mechanism of chromophore formation // FEBS letters, 592 (2018), 9; 1516-1523 doi:10.1002/1873-3468.13051 (međunarodna recenzija, pismo, znanstveni)
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Naslov
Non-fluorescent mutant of green fluorescent protein sheds light on the mechanism of chromophore formation
Autori
Bartkiewicz, Małgorzata ; Kazazić, Saša ; Krasowska, Joanna ; Clark, Patricia L. ; Wielgus-Kutrowska, Beata ; Bzowska, Agnieszka
Izvornik
FEBS letters (0014-5793) 592
(2018), 9;
1516-1523
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pismo, znanstveni
Ključne riječi
chromophore maturation ; green fluorescent protein ; ms/ms spectrometry ; S65T mutant ; S65T/G67A mutant
Sažetak
The mechanism of green fluorescent protein (GFP) chromophore formation is still not clearly defined. Two mechanisms have been proposed: cyclisation–dehydration–oxidation (Mechanism A) and cyclisation–oxidation–dehydration (Mechanism B). To distinguish between these mechanisms, we generated a non–fluorescent mutant of GFP, S65T/G67A-GFP. This mutant folds to a stable, native-like structure but lacks fluorescence due to interruption of the chromophore maturation process. Mass spectrometric analysis of peptides derived from this mutant reveal that chromophore formation follows only mechanism A, but that the final oxidation reaction is suppressed. This result is unexpected within the pool of examined GFP mutants, since for the wild-type GFP, there is strong support for mechanism B.
Izvorni jezik
Engleski
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
