Naslov
Vancomycin and gentamicin interference on results of clinical chemistry tests on Abbott Architect c8000 analyzer

Autori
Nikolac, Nora ; Brenčić, Tina ; Topić, Anita

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Clinical Chemistry and Laboratory Medicine / - , 2017, A50-A50

Skup
4th EFLM-BD European Conference on Preanalytical Phase – Improving Quality of Preanalytical Phase through inovation

Mjesto i datum
Amsterdam, Nizozemska, 24.03.2017. - 25.03.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
interference, preanalytical phase, vancomycin, gentamicin

Sažetak
Toxic concentrations of vancomycin and gentamicin can be observed in nephrology patients. Little is known on influence of high drug concentrations on results of clinical chemistry tests. In this work we aimed to investigate interferences of vancomycin and gentamicin on results of 25 commonly measured biochemistry tests on Abbott Architect c8000. Study was carried out in University Department of Chemistry, Medical School University Hospital Sestre Milosrdnice (Zagreb, Croatia) from June to August 2016. For each drug, 10 aliquotes of serum pool were prepared. In order to cover toxic concentrations, pool serum samples were spiked with drugs to obtain: 0-50 µg/mL of gentamicin and 0-200 µg/mL of vancomycin. Measurements of biochemistry tests were done in duplicate on the analyzer Architect c8000 and drug concentrations was measured on the analyzer Architect i2000 SR (both Abbott, Illinois, USA). For each tested concentration, bias was calculated against the initial measurement. Acceptance criteria were defined as measurement uncertainty of the commercial control with value close to the measured range of the pool sample. For gentamicin interference testing, all bias values were bellow established acceptance criteria. For vancomycin, significant changes were observed for potassium, direct bilirubin and IgA. The potassium bias at the highest vancomycin concentration of 204.4 µg/mL exceeded acceptance criteria (bias=-4.5%, acceptable 4%). For direct bilirubin significant bias was detected already at low vancomycin concentration of 2.98 µg/mL (bias=9.7%, acceptable 8%). For IgA, no apparent trend was observed, and exceeded bias values are attributed to the increased method imprecision. Gentamicin didn’t interfere with the results of clinical chemistry tests. Direct bilirubin concentration is falsely increased in the presence of vancomycin, while potassium is affected at high concentrations.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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