Pregled bibliografske jedinice broj: 982872
Proteomic Analysis of the Venom from the Ruby Ant Myrmica rubra and the Isolation of a Novel Insecticidal Decapeptide
Proteomic Analysis of the Venom from the Ruby Ant Myrmica rubra and the Isolation of a Novel Insecticidal Decapeptide // Insects, 10 (2) (2019), 42; 10020042, 17 doi:10.3390/insects10020042 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 982872 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Proteomic Analysis of the Venom from the Ruby Ant Myrmica rubra and the Isolation of a Novel Insecticidal Decapeptide
Autori
Heep, John ; Klaus, Alica ; Kessel, Tobias ; Seip, Maximilian ; Vilcinskas, Andreas ; Skaljac, Marisa
Izvornik
Insects (2075-4450) 10 (2)
(2019), 42;
10020042, 17
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
mass spectrometry ; LC-MS ; Formicidae ; Myrmicinae ; European fire ant ; antimicrobial peptide ; peptide toxin ; bio-insecticide ; aphids ; Acyrthosiphon pisum
Sažetak
Ants are a biodiverse group of insects that have evolved toxic venom containing many undiscovered bioactive molecules. In this study, we found that the venom of the ruby ant Myrmica rubra is a rich source of peptides. LC-MS analysis revealed the presence of 142 different peptides varying in molecular weight, sequence length, and hydrophobicity. One of the most abundant peaks was selected for further biochemical and functional characterization. Combined Edman degradation and de novo peptide sequencing revealed the presence of a novel decapeptide (myrmicitoxin) with the amino acid sequence NH2- IDPKLLESLA-CONH2. The decapeptide was named U- MYRTX-MRArub1 and verified against a synthetic standard. The amidated peptide was tested in a synthetic form to determine the antimicrobial activity towards the bacterial pathogens and insecticidal potential against pea aphids (Acyrthosiphon pisum). This peptide did not show antimicrobial activity but it significantly reduced the survival of aphids. It also increased the sensitivity of the aphids to two commonly used chemical insecticides (imidacloprid and methomyl). Since ant venom research is still in its infancy, the findings of this first study on venom peptides derived from M. rubra highlight these insects as an important and rich source for discovery of novel lead structures with potential application in pest control.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Poljoprivreda (agronomija), Biotehnologija
POVEZANOST RADA
Projekti:
Research and the Arts (HMWK) via the “LOEWE Center for Insect Biotechnology and Bioresources”
Hessen State Ministry of Higher Education
Ustanove:
Institut za jadranske kulture i melioraciju krša, Split
Profili:
Marisa Škaljac
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
