DNA damage in rat kidneys and liver upon subchronic exposure to single and combined ochratoxin A and citrinin

Rašić, Dubravka; Želježić, Davor; Kopjar, Nevenka; Kifer, Domagoj; Šegvić Klarić, Maja; Peraica, Maja

doi:10.3920/WMJ2018.2399

Pregled bibliografske jedinice broj: 982137

DNA damage in rat kidneys and liver upon subchronic exposure to single and combined ochratoxin A and citrinin

Rašić, Dubravka; Želježić, Davor; Kopjar, Nevenka; Kifer, Domagoj; Šegvić Klarić, Maja; Peraica, Maja

DNA damage in rat kidneys and liver upon subchronic exposure to single and combined ochratoxin A and citrinin // World Mycotoxin Journal, 12 (2019), 2; 163-172 doi:10.3920/WMJ2018.2399 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 982137 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
DNA damage in rat kidneys and liver upon subchronic exposure to single and combined ochratoxin A and citrinin

Autori
Rašić, Dubravka ; Želježić, Davor ; Kopjar, Nevenka ; Kifer, Domagoj ; Šegvić Klarić, Maja ; Peraica, Maja

Izvornik
World Mycotoxin Journal (1875-0710) 12 (2019), 2; 163-172

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
ochratoxin A, citrinin, hOGG1-modiffied comet assay, oxidative stress, resveratrol

Sažetak
The study was aimed to check whether ochratoxin A (OTA) and citrinin (CIT) increase DNA damage in the kidney and liver of male Wistar rats (alkaline comet assay) ; clarify the oxidative nature of DNA damage (hOGG1-modified comet assay) ; and verify whether resveratrol (RSV) could ameliorate OTA+CIT- induced genotoxicity. Rats were treated orally with OTA (0.125 and 0.250 mg kg-1 b.w.) and CIT (2 mg kg-1 b.w.), OTA+CIT combinations and OTA+CIT+RSV (0.250+2+20 mg kg-1 b.w.) for 21 days. Both alkaline and hOGG1-modified comet assay showed that DNA damage was more severe in rat kidneys than in liver following mycotoxin treatment. Alkaline comet assay revealed a higher intensity of DNA damage, particularly as measured by tail intensity in the kidneys. Both tail length and tail intensity were OTA dose-dependent, but in combined OTA+CIT treatment these values were similar to CIT alone and lower than in animals treated with single OTA, possibly due to induction of apoptosis. hOGG1-modified comet showed that OTA+CIT evoked greater oxidative DNA damage than single mycotoxins. RSV did not reduce DNA damage measured by alkaline comet assay, but hOGG1-modified comet showed that RSV ameliorated OTA+CIT genotoxicity in the kidneys. Apart from oxidative stress, other mechanisms of DNA damage are involved in OTA and CIT genotoxicity. In rat kidneys RSV can reduce but not overcome oxidative DNA damage induced by combined OTA and CIT.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita, Farmacija

POVEZANOST RADA

Projekti:
HRZZ-IP-2014-09-5982 - Štetni učinci pojedinačnih i kombiniranih mikotoksina Aspergillus vrsta (MycotoxA) (Šegvić Klarić, Maja, HRZZ - 2014-09) ( CroRIS)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut za medicinska istraživanja i medicinu rada, Zagreb

Profili:

Domagoj Kifer (autor)