Naslov
Evidence for asymmetry of alkaline phosphatase from E.coli

Autori
Stjepan Orhanović ; Maja Pavela-Vrančić ; Mirna Flogel-Mršić

Izvornik
Acta pharmaceutica (1330-0075) 44 (1994), 1; 87-95

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
alkaline phosphatase ; asymmetry ; curve fitting

Sažetak
The steady-state kinetics of alkaline phosphatase from E. coli performed with pNPP as a substrate have been investigated. The enzyme shows deviation from Michaelis-Menten kinetics giving concave down Hanes plots. In the presence of a competitive substrate analogue, this effect become more pronounced. In an attempt to interpret the experimental data, non-linear regression fitting was applied to equations describing models, based on either negative cooperative interactions between subunits or independent nonequivalent active sites. The results obtained with pNPP as a substrate could not clearly differentiate between an allosteric and asymmetric model. however increased deviations observed in the presence of a substrate analogue could only be substantiated by a model assuming inherently nonequivalent subunits.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA