Naslov
Determination of oxidative stress upon high voltage electrical discharge (HVED) plasma on Escherichia coli MG 1655

Autori
Stulić, Višnja ; Vukušić, Tomislava ; Režek Jambrak, Anet ; Jurić, Slaven ; Herceg, Zoran

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
7th Central European Symposium on Plasma Chemistry, Sveti Martin na Muri, Croatia / Milošević, Slobodan ; Krstulović, Nikša - Zagreb, 2017, 39-39

ISBN
978-953-7666-16-3

Skup
7th Central European Symposium on Plasma Chemistry

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 03.09.2017. - 07.09.2017

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Escherichia coli, cold plasma, oxidative stress

Sažetak
High voltage electrical discharge (gas and liquid) plasmas are new rapidly developing techniques used for food and water decontamination [1]. Though, one of the main questions to answer are effectiveness of this method and full understanding of cell inactivation mechanisms after plasma treatments. This study investigates the influence of high voltage gas phase plasma treatment on several parameters: inactivation rate of Escherichia coli K12 MG1655, cells recovery, cellular leakage, biofilm formation and influence of pH and generated H2O2 species on inactivation of the Gram negative bacteria. Bacterial solution of Escherichia coli K12 was treated in a glass reactor with a point-to- plate electrode configuration. Four independent variables: electrical conductivity, polarity, time and frequency were chosen in order to determine their influence on listed parameters. Statistical analyses were obtained to optimize conducted treatments. Treated samples gave positive reaction to catalase activity, as a response to oxidative stress. Biofilm formation was not completely reduced, as all the samples gave increase in percentage of recovery. Also with a longer treatment time increase in cellular leakage was observed. Synergistic effects of polarity, treatment time, solution conductivity, pH and concentration of free radicals could have influence on inactivation rate of E. coli, but further optimization of method is needed to understand why the recovery of cell occurs.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

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