Naslov
The perinatal mild hypoxic brain lesion in rats causes changes in perineuronal nets morphology and impaired learning behavior
(The perinatal mild hypoxic brain lesion in rats causes changes in perineuronal nets morphology and impaired learning behavior)

Autori
Trnski, Sara ; Ilić, Katarina ; Nikolić, Barbara ; Orešković, Darko ; Habek, Nikola ; Hranilović, Dubravka ; Jovanov Milošević, Nataša

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Controlling neuronal plasticity - developmental disorders and repair : book of abstracts / - , 2018, 45-45

Skup
Controlling neuronal plasticity - developmental disorders and repair

Mjesto i datum
Prag, Češka Republika, 06.12.2018. - 07.12.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Wisteria floribunda agglutinin ; parvalbumin ; hypoxia ; brain development

Sažetak
This study investigates the changes in expression of perineuronal nets (PNN’s) and behavioral deficits after mild perinatal hypoxic brain lesion in rats, to serve for the research targeting the fetal hypoxic brain lesions occurring during midgestation (23-32 weeks post-conception) in humans. In the present study, Wistar rats (9 females and 10 males) were randomly divided into hypoxic and control group on postnatal day 1 (P1) when hypoxia was induced in a warm (≈ 25°C) hypobaric chamber (Atm 350mmHg, pO273mmHg) during 2 hours, while controls were kept in normal housing conditions. Behavioral tests were performed at P30 and P70 using the open field, hole board, social choice, and T-maze tests. Samples of brain tissue from adult animals (P105) were used for histochemical examination of cytoarchitectonics (Nissl staining), interneurons (parvalbumin immunohistochemistry) and perineuronal nets (Wisteria floribunda agglutinin, histochemistry). After short-term perinatal rat brain injury, structural cerebral cytoarchitectonics, as well as the laminar and structural organization of the telencephalon, motor and socialization patterns were preserved. However, changes in morphology, number, and distribution of the parvalbumin- immunoreactive neurons and perineuronal nets, distinct in different regions of the telencephalon were observed. Furthermore, treated animals also shown significantly impaired learning behavior. In conclusion, the perinatal mild hypoxic brain lesion in rats leads to consistent disturbances in brain connectivity related to cognitive processes, that mimic perinatal mild post-hypoxia condition in humans. Further characterization and evaluation of this brain injury model, on molecular, cytological and connectivity levels, is needed to disclose developmental disturbances that are not compensated after the provoked hypoxia and therefore lead to cognitive deficits. Acknowledgement: KK.01.1.1.01.0007, CoRE – Neuro and Croatian Science Foundation.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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