Naslov
Hidden carbapenem resistance in OXA-48 and extended- spectrum β-lactamase-positive Escherichia coli

Autori
Tomić Paradžik , Maja ; Drenjančević , Domagoj ; Presečki-Stanko , Aleksandra ; Kopić , Jasminka ; Talapko , Jasminka ; Zarfel , Gernot ; Bedenić , Branka

Izvornik
Microbial drug resistance (1076-6294) 25 (2019), 5; 696-702

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Escherichia coli ; β-lactamases ; ESBL ; carbapenemases ; OXA-48

Sažetak
The purpose of this study was to report the identification OXA-48 carbapenemase in seven extended-spectrum β-lactamase (ESBL)-positive Escherichia coli clinical isolates, fully susceptible to all carbapenems by disk diffusion and E-test methods, but with borderline minimal inhibitory concentration (MIC) values of ertapenem. This report points to the necessity for determination of carbapenem MICs in ESBL-positive E. coli isolates and additional phenotypic testing for carbapenemases in all isolates with borderline ertapenem MIC defined by EUCAST. The isolates showed a high level of resistance to expanded- spectrum cephalosporins because of the production of an additional ESBL belonging to CTX-M family. All isolates and their respective tranconjugants were found to possess L plasmid. Pulsed-field gel electrophoresis analysis revealed two clusters containing highly related isolates. The global spread of multidrug-resistant E. coli should be monitored closely because of the ability of isolates to rapidly obtain additional antibiotic resistance traits such as plasmid-mediated OXA-48 genes.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA