Naslov
Toxicology and antitumor evaluation of two novel monomethine cyanine derivatives in vivo

Autori
Mišković, Katarina ; Belovari, Tatjana ; Rajc, Jasmina ; Šerić, Vatroslav ; Stojković, Ranko ; Piantanida, Ivo ; Baus Lončar, Mirela ; Glavaš-Obrovac, Ljubica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10th ISABS Conference on Forensic and Anthropologic Genetics and Mayo Clinic Lectures in Individualized Medicine : Program and abstracts / Primorac, Dragan ; Schanfiled, Moses ; Vuk-Pavlović, Stanimir ; Kayse, Manfred ; Ordog, Tamas - Zagreb : International Society for Applied Biological Sciences (ISABS), 2017, 255-255

ISBN
978-953-57695-2-1

Skup
10th ISABS Conference on Forensic and Anthropologic Genetics and Mayo Clinic Lectures in Individualized Medicine

Mjesto i datum
Dubrovnik, Hrvatska, 19.06.2017. - 24.06.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antitumor evaluation ; monomethine cyanine derivatives

Sažetak
The area of small, biological active molecules that interact with specific cell compartment are in focus of today’s science. Small molecules with low toxicity and specific mode of interaction are interesting in the area of molecular therapy especially for cancer treatment and future of personalised medicine. One, very interesting biologically active group of small molecules are monomethine cyanine dyes (MCDs) with preferable binding mechanism in to a minor groove of DNA. In this study we evaluate toxicological and antitumor effect of two, new monomethine cyanine derivatives designated as monomethine cyanine derivatives no. 4 and no.8. Highly inbreeded hybrid mouse WT strain created as combination of 129/Sv and C57BL/6j were used for acute and chronic toxicology study and BalbC female mice were used for antitumor study. Four mice for each sex were used in experimental groups. Haematological (E, L, Hg, HE, MCV, MCV, MCHC, Plt ) and biochemical (AST, ALT, AP, LDH, glucose, urea, creatinine, Na, K) parameters, histomorfological analysis of body organs (brain, hart, spleen, bone, stomach, intestine, colon, kidney, liver, lungs) and size of grown tumour are measured as tool for in vivo effect. Obtained results are analysed by multifactorial variance analysis (MANOVA) and Fisher LSD test in STATISTICA 8.0 program. One time application (7mg/kg) with MCD 4 in evaluation of acute toxicity resulted with increased levels of AST, ALT and LDH and decreased glucose. Chronic toxicity (3x7mg/kg) because of the prolonged exposure and multiple MCD 4 application did not show significant change on the male mice. MCD 4 applied to the female mice raised levels of AST, ALT and LDH and decreases ALP. Results points to enhanced sensibility of female mice compared to males in prolonged time of exposure to MCD 4. Chronic toxicity caused by the MCD 8 did not point to any significantly altered biochemical parameter no matter the sex of the mouse. Results pointed to stronger MCD 4 toxic effect in chronic exposure to female in comparison to male mice. There were no significant suppression of implanted tumour regardless to applied concentration of tested derivatives.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Interdisciplinarne prirodne znanosti, Temeljne medicinske znanosti

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