Naslov
Efficient micropropagation and rooting of Micromeria croatica (Pers.) Schott (Lamiaceae)

Autori
Kereša, Snježana ; Andrijanić, Zoe ; Kremer, Dario ; Habuš Jerčić, Ivanka ; Barić, Marijana ; Batelja Lodeta, Kristina ; Bolarić, Snježana ; Bošnjak Mihovilović, Anita

Izvornik
Poljoprivreda (Osijek) (1330-7142) 24 (2018), 2; 27-33

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
in vitro propagation ; Micromeria croatica ; plant growth regulators

Sažetak
Micromeria croatica is an endangered species with a great potential for use as a medical as well as ornamental plant. For commercial use, an efficient propagation protocol is required. The aims of this study were to establish an efficient protocol for micropropagation and rooting of M. croatica. A number of factors were tested including the influence of 6-benzylaminopurine (BAP) and 1- naphthaleneacetic acid (NAA) at different concentrations as well as the effect of explant orientation and topophysis on shoot proliferation. Rooting was investigated in agar-based medium supplemented with indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) at various concentrations. Media with 1 and 2 mg/L of BAP produced the highest number of shoots/explant. However, the lower concentration of BAP should be recommended due to longer shoots without any sign of hyperhydration. Explant orientation and node position showed equal capability for shoot proliferation. Importantly, 100% efficiency of rooting was achieved in an agar-based rooting medium. Acclimatisation exceeded 90%. Taken together, an efficient protocol for micropropagation and rooting of M. croatica has been established for future production.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija)

POVEZANOST RADA