Naslov
Istraživanje interakcija cijaninskih boja s polinukleotidima spektroskopskim i termoanalitičkim metodama
(Study of cyanine dyes interactions with polynucleotides using spectroscopic and thermoanalytical methods of analyses)

Autori
Rožman, Andrea

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, doktorska disertacija

Fakultet
Prirodoslovno-matematički fakultet, Kemijski odsjek

Mjesto
Zagreb

Datum
13.12

Godina
2018

Stranica
107

Mentor
Piantanida, Ivo

Ključne riječi
cirkularni dikroizam ; DNA i RNA prepoznavanje ; fluorescentni markeri ; metom inducirana agregacija
(circular dichroism ; DNA and RNA recognition ; fluorescent markers ; target-aggregating dyes)

Sažetak
Introduction of chloro-substituent at cyanine chromophore of well-known DNA stains TOTO and YOYO resulted in a significant difference of DNA and RNA recognition in respect to parent compounds (TOTO ; YOYO), as well as in respect to corresponding monomers (Cl-TO and Cl-YO). Studied novel compounds showed strong, submicromolar affinity to all ds-polynucleotides, with minor differences in binding constant between various DNA and RNA. The Cl-YOYO revealed a specific fluorimetric recognition of ds-RNA in respect to ds-DNA by the fluorimetric response at 570 nm, which was attributed to particular aggregation property of dye, not present in corresponding Cl-TOTO analogue. The Cl-TOTO, unlike YO-analogue, showed ICD band at 520 nm only upon binding to alternating DNA sequences (both, GC-GC and AT-AT), which was not observed for homo-DNA and homo-RNA. Such recognition pattern was attributed to structural differences between polynucleotides, whereby only alternating-DNAs have a typical B-helical structure in which Cl-TOTO molecule could self-fold to give particular ICD band at 520 nm. Thus, both Cl-analogues show intriguing novel recognition properties in respect to parent TOTO and YOYO, which combined with negligible cytotoxicity offer potential for development of novel dyes for biochemical and biomedical applications.

Izvorni jezik
Hrvatski

Znanstvena područja
Kemija

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