Naslov
Variability of Salivary Oxidative Stress Markers in Healthy Subjects

Autori
Alajbeg, Iva ; Roglić Dunja ; Illeš, Davor ; Vuletić, Lea ; Andabak Rogulj, Ana ; Lapić, Ivana ; Knezović Zlatarić, Dubravka ; Badel, Tomislav ; Alajbeg, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Journal of Dental Research / Giannobile, William V - : International Association for Dental Research, 2017, 1648-1648

Skup
IADR/AADR/CADR General Session

Mjesto i datum
San Francisco (CA), Sjedinjene Američke Države, 20.03.2017. - 25.03.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
salivary oxidative stress ; healthy population

Sažetak
Objectives: Little data exist regarding normal variations for salivary oxidative stress (OS) markers in healthy population. The aim of this study was to determine the normal intra- and inter-day variations of salivary OS markers in healthy adult individuals. Methods: Unstimulated saliva from 15 healthy adults was collected twice daily (7:00 AM and 5:00 PM) on three consecutive days. Salivary levels of OS markers, including glutathione peroxidase (GPX), superoxide dismutase (SOD), total antioxidant capacity (TAC), 8- hydroxydeoxyguanosine (8-0HdG) and uric acid (UA) were determined. Results: Concentrations of most measured markers did not differ significantly during the day ; only SOD showed significant intra-day variations, with significantly higher values in the morning compared to the afternoon measurements (F=12.72, p=0.003). A within subjects MANOVA did not show the significant influence of “sampling day” as a factor affecting the variability of salivary OS markers (P>0.05). The highest intraclass correlations (ICC’s) were obtained for UA and 8- 0HdG (0.871 and 0.858 respectively). Conclusions: Salivary OS markers can be measured reproducibly over time, since no significant inter-day variability for all measured markers was observed. Moreover, concentrations of most measured markers did not differ significantly during the day. Only SOD showed significant intra- day variations. The excellent reliability obtained for UA and 8- 0HdG indicates that those markers could potentially be more specific in terms of distinguishing patient population from the healthy controls.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Dentalna medicina

POVEZANOST RADA