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Tihomir Kovač
Bojan Šarkanj
Biljana Crevar
Marija Kovač
Ante Lončarić
Ivica Strelec
Chibundu N. Ezekiel
Michael Sulyok and Rudolf Krska
VIS light illumination
Aspergillus flavus
mycotoxins
mycelial growth
oxidative status modulation
aflatoxin production
optogenetics
Aspergillus flavus is the most important mycotoxin-producing fungus involved in the global episodes of aflatoxin B1 contamination of crops at both the pre-harvest and post-harvest stages. However, in order to effectively control aflatoxin contamination in crops using antiaflatoxigenic and/or antifungal compounds, some of which are photosensitive, a proper understanding of the photo-sensitive physiology of potential experimental strains need to be documented. The purpose of the study is therefore to evaluate the effect of visible (VIS) light illumination on growth and conidiation, aflatoxin production ability and modulation of A. flavus oxidative status during in vitro experiment. Aflatoxigenic A. flavus strain was inoculated in aflatoxin-inducing YES media and incubated under three different VIS illumination regimes during a 168 h growth period at 29 C. VIS illumination reduced A. flavus mycelia biomass yield, both during growth on plates and in liquid media, promoted conidiation and increased the aflatoxin production. Furthermore, aflatoxin production increased with increased reactive oxidative species (ROS) levels at 96 h of growth, confirming illumination-driven oxidative stress modulation activity on A. flavus cells.
Aspergillus flavus NRRL 3251 Growth, Oxidative Status, and Aflatoxins Production Ability In Vitro under Different Illumination Regimes
application/pdf
2018-12-10T10:54:06+01:00
LaTeX with hyperref package
2018-12-10T11:12:38+01:00
VIS light illumination; Aspergillus flavus; mycotoxins; mycelial growth; oxidative status modulation; aflatoxin production; optogenetics
pdfTeX-1.40.18
This is pdfTeX, Version 3.14159265-2.6-1.40.18 (TeX Live 2017/W32TeX) kpathsea version 6.2.3
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