Naslov
MORPHOLOGY AND PHYLOGENY OF PICOEUKARYOTES AND PLANKTONIC PENNATE DIATOMS IN THE MIDDLE AND SOUTH ADRIATIC SEA

Autori
Mucko, Maja

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, doktorska disertacija

Fakultet
Prirodoslovno-matematički fakultet

Mjesto
Zagreb

Datum
06.07

Godina
2018

Stranica
52

Mentor
Ljubešić, Zrinka

Ključne riječi
picoeukaryotes, planktonic pennate diatoms, morphology, phylogeny

Sažetak
The smallest protists, generally called picoeukaryotes, and planktonic pennate diatoms have received limited attention in world oceans, although they are numerous and important for primary production, biogeochemical cycles and for carbon injection into the deep ocean. In this thesis, represented through seven scientific publications, cultivated planktonic pennate diatoms and picoeukaryotes were investigated with traditional methods (light and electron microscopy, morphometry) and with molecular analyses, including multi-gene phylogenies, and were identified as genera Entomoneis, Pseudo-nitzschia, Haslea and Picochlorum. Detailed investigations of genus Entomoneis resulted with description of seven new species. General picoeukaryotic diversity was addressed by eDNA amplicon sequencing V4 variable region of nuclear 18S rRNA gene. Obtained results identified 95% of the picoeukaryotic community as hetero- or mixotrophic, mostly belonging to parasitic dinoflagellates and radiolarians, while only 5% of the community was represented with photoautotrophic picoeukaryotes belonging to classes of green algae, haptophytes, stramenopiles and cryptophytes. One cultivated Picochlorum sp. strain was subjected to growth rate experiment and pigment and lipid analyses, resulting in interesting and promising data for future biotechnological studies.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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