Naslov
Transcriptomic and ultrastructural changes of the Atlantic bluefin tuna Thunnus thynnus (Linnaeus, 1758) gill epithelium infected with Didymosulcus katsuwonicola (Pozdnyakov, 1990).

Autori
Trumbić, Željka ; Taggart, John Bernard ; Bron, James E ; Bočina, Ivana ; Mladineo, Ivona

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book of the 17th International Conference on Diseases of Fish and Shellfish / Mladineo, Ivona - , 2015, 125-125

Skup
17th EAFP International Conference on Diseases of Fish and Shellfish

Mjesto i datum
Las Palmas de Gran Canaria, Španjolska, 07.09.2015. - 11.09.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Thunnus thynnus ; Transcriptome ; Didymosulcus katsuwonicola, microarray, TEM

Sažetak
In the Atlantic bluefin tuna Thunnus thynnus (Scombridae), didymozoids, considered one of the most taxonomically complex digenean families, inhabit all conceivable niches. They encyst mostly in pairs in a connective tissue capsule of varying size and thickness formed mutually by parasite and the host. Didymosulcus katsuwonicola (syn. Didymocystis wedli) is the most abundant didymozoid species infecting farmed T. thynnus with gills as a predilected site of infection. In most cases infections terminate without any gross pathology, characterised by a significant decrease of cysts per host after couple of months of tuna rearing, however under certain conditions inflammatory and necrotic changes in the gill tissue may be induced. This dynamics is explained as the net result of tuna physiology and specifics of the rearing environment, making didymozoid infection an interesting model for parasite-host interaction study. The main goal of our study was to assess the response of tuna gill tissue parasitised by D. katsuwonicola at the trasncriptomic level and compare it to the ultrastructural changes inferred by transmission electron microscopy (TEM). Punch biopsies were collected from infected and uninfected T. thynnus gills at harvest, at the end of a two-year rearing cycle. Samples were prepared for transcriptomic profiling by DNA microarrays and TEM according to standardized protocols. T. thynnus-specific custom 15K Agilent oligo microarray was employed in this study. The transcriptional profiling revealed moderate gene regulation in both directions with fold changes ranging from -10.38 to 4.56 (N=768 statistically selected features). Pathway analyses based on KEGG sets showed the perturbations of components of innate immunity, complement and coagulation cascades, as well as endocrine, digestive and nervous functional pathways. Micrographs showed a heterogeneous cell population at the host- parasite border. Numerous transiting vesicles were recorded in didymozoid’s tegument in direct contact with host’s loose collagen connective tissue capsule comprising fibrocytes, fibroblasts, eosinophilic- granulated mast cells, eosinophils and plasma cells. The capsule encompassed a nerve composed of three neuronal axons, numerous anastomosing capillaries and was overlaid with multilayered squamous epithelium abundant with mucous goblet cells. The findings elucidate the utility of a molecular and ultrastructural approach in a host-parasite interaction study.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina, Biotehnologija

POVEZANOST RADA