Naslov
Reactor and microreactor performance and kinetics of the aldol addition of dihydroxyacetone to benzyloxycarbonyl-N-3-aminopropanal catalyzed by D-fructose-6-phosphate aldolase variant A129G

Autori
Sudar, Martina ; Findrik, Zvjezdana ; Szekrenyi, Anna ; Clapés, Pere ; Vasić-Rački, Đurđa

Izvornik
Chemical engineering communications (0098-6445) 206 (2019), 7; 927-939

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
FSA ; kinetic parameters ; kinetic model ; batch reactor ; microreactor

Sažetak
D-Fagomine is an iminosugar found in nature that lowers blood glucose peak after meal and reduces fat-induced weight gain and insulin resistance. The immediate precursor of D- fagomine is accessible straightforward via D- fructose-6-phosphate aldolase catalyzed aldol addition of dihydroxyacetone to Cbz-N-3- aminopropanal (Cbz = benzyloxycarbonyl). In this work, the performance and kinetics of the abovementioned reaction catalyzed by FSA A129G variant was studied. The reaction was investigated in two reactors ; batch reactor and microreactor, and kinetic parameters were estimated from the steady state experiments in the batch reactor. This enzyme has improved stability, better Km value for Cbz-N-3- aminopropanal and lower retro-aldol activity in comparison with previously studied aldolase variants FSA A129S and A129S/A165G. Mathematical model for both reactors was developed and validated experimentally.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

POVEZANOST RADA