Naslov
Micro- and nano-mechanics of osteoarthritic cartilage: The effects of tonicity and disease severity

Autori
Moshtagh, PR ; Pouran, B ; van Tiel, J ; Rauker, J ; Zuiddam, MR ; Arbabi, V ; Korthagen, NM ; Weinans, H ; Zadpoor, AA

Izvornik
Journal of the Mechanical Behavior of Biomedical Materials (1751-6161) 59 (2016); 561-571

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Articular cartilage, Osmolality, Nano-stiffness

Sažetak
The present study aims to discover the contribution of glycosaminoglycans(GAGs)and collagen fibers to th emechanical properties of the osteoarthritic(OA) cartilage tissue.We used nanoindentation experiments to underst and the mechanical behavior of mild and severe osteoarthritic cartilage at micro-and nano- scale at different swelling conditions. Contrast enhanced micro- computedtomography(EPIC-μCT) was used to confirm that mild OA speciments had significantly higher GAGs content compared to severe OA specimens.In micro-scale, the semi- equilibrium modulus of mild OAspecimens significantly dropped after immersion in a hypertonic solution and at nano-scale, the histograms of the measured elastic modulus revealed three to four components.Comparing the peaks with those observed for healthy cartilage in a previous study indicated that the first and third peaks represent the mechanical properties of GAGs and the collagen network.The third peak shows considerably stiffer elastic modulus for mild OA samples as compared to the severe OA samples in isotonic conditions.Furthermore, this peak clearly dropped when the tonicity increased, indicating the loss of collagen (pre-) stress in the shrunk specimen.Our observations support the association of the third peak with the collagen network.However, our results did not provide any direct evidence to support the association of the first peak with GAGs.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA