Naslov
Arabidopsis MATH-BTB proteins are involved in RNA- directed DNA methylation

Autori
Jagić, Mateja ; Škiljaica, Andreja ; Bauer, Nataša ; Leljak-Levanić, Dunja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
BOOK OF ABSTRACTS OF THE 13th CROATIAN BIOLOGICAL CONGRESS / Kružić, Petar ; Caput Mihalić, Katarina ; Gottstein, Sanja ; Pavoković, Dubravko ; Kučinić, Mladen - Zagreb : Hrvatsko biološko društvo, 2018, 54-55

Skup
13. Hrvatski biološki kongres

Mjesto i datum
Poreč, Hrvatska, 19.09.2018. - 23.09.2018

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
MATH-BTB, Arabidopsis thaliana, protein-protein interactions, RNA-directed DNA methylation

Sažetak
Arapidopsis BPM proteins belong to MATH-BTB protein family. MATH-BTB proteins partake in many developmental processes in plants and animals and are known as key regulators of the cell cycle. Arabidopsis genome contains six genes encoding for BPM proteins (BPM1-6). BPM proteins bind members of several transcription factor families targeting them for ubiquitination by Cullin3 E3 ligase. Our preliminary results showed BPM1 interaction with DMS3 and RDM1, important components of RNA-directed DNA methylation (RdDM). Still, the role of BPM proteins in DNA methylation remains unknown. To elucidate an involvement of other Arabidopsis MATH-BTB proteins (BPM2-6) in RdDM, interactions with DMS3 and RDM1 were examined in yeast two hybrid system. Additionally, truncated versions of the BPM1 missing an individual domain were used to test domain specificity for interaction with DMS3 and RDM1. All BPM proteins show interactions with both investigated components of RdDM. BTB domain of BPM1 protein proved to be the most important for interaction with RDM1. Results obtained here point to a role of BPM proteins in regulation of DNA methylation that will be further investigated.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA