Naslov
DNA methylation in head and neck cancer and oral lesions

Autori
Milutin Gašperov, Nina ; Božinović, Ksenija ; Sabol, Ivan ; Grce, Magdalena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Translating Science of Medicine - Targets and Therapeutics, Fifth Meeting of the Croatian Association for Cancer Research with International Participation : Poster presentations, in Libri oncologici, Vol. 46 No. Supplement 1 / Ozretić, Petar ; Levanat, Sonja - Zagreb : Klinički bolnički centar Sestre milosrdnice, 2018, 27-27

Skup
5th Meeting of the Croatian Association for Cancer Research with International Participation: Translating Science to Medicine "Targets and Therapeutics" (HDIR-5)

Mjesto i datum
Zagreb, Hrvatska, 08.11.2018. - 10.11.2018

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
head and neck cancer ; oral lesions ; DNA methylation

Sažetak
Head and Neck Squamous Cell Carcinoma (HNSCC) is the 7th most frequent malignancy in the world, with a very low 5-year survival. It is known that the main risk factors associated with this carcinoma are excessive tobacco and alcohol consumption together with high-risk types of human papillomavirus (HPV). Still, there is a strong need to find new, good biomarkers of this disease. The most appropriate biomarkers would be those that would point out changes in epithelial cells before carcinoma occurrence. DNA methylation is irreversible change that inhibits gene expression. Altered DNA methylation is one of the possible factors associated with the HNSCC development. The presumption is that epigenetic biomarkers, such as methylated genes could point to changes even before they can be clinically detected. The model on which we explore those changes of DNA methylation are normal oral mucosa tissue, potentially malignant lesions of the oral mucosa, oral lichen planus (OLP) and oral lichenoid lesions (OLL) and HNSCC tissue. OLP and OLL are difficult to distinguish clinically and histopathologically, so early detection and distinguishing by epigenetic tools would be of great importance in further treatment. Herein, we will discuss DNA methylation profiling of different diagnosis by Infinium MethylationEPIC BeadChip array and pyrosequencing methods. The main goal was to reveal DNA methylation differences in normal oral samples, possible in oral lesions, and in HNSCC samples. The results obtained with Infinium MethylationEPIC BeadChip array showed a panel of gene promoters significantly methylated in HNSCC samples in comparison to normal samples, mainly of genes related with receptor and transmembrane functions and transcription activity. Some of them are included in cell cycle, cellular growth, transformation, apoptosis and autophagy. The group of hypomethylated gene promoters in HNSCC, in comparison to normal oral mucosa, and lesions, was mainly involved in immune response and transcriptional regulation.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA