Naslov
Activation of MAPKs in bronchial-epithelial NCI-H292 cells co-treated with extracellular Hsp70 and bacterial components or cigarette smoke extract

Autori
Somborac Bačura, Anita ; Hulina, Andrea ; Grdić Rajković, Marija ; Čeri, Andrea ; Popović-Grle, Sanja ; Rumora, Lada

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
ERS International Congress 2018 / - : European Respiratory Society, 2018, 4084-4084

Skup
28th International Congress of the European Respiratory Society (ERS 2018)

Mjesto i datum
Pariz, Francuska, 15.09.2018. - 19.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
chronic obstructive pulmonary disease ; extracellular Hsp70 ; cigarette smoke ; LPS ; LTA ; MAPKs ; NCI-H292 cell line

Sažetak
Introduction: The main etiologic factor for chronic obstructive pulmonary disease (COPD), which is characterized by chronic airway inflammation, is cigarette smoking. Patients with COPD are susceptible to bacterial infections causing acute exacerbations. Extracellular Hsp70 (eHsp70) can act as damage-associated molecular pattern and activate immune cells via Toll-like receptors 2 and 4. Aims and objectives: The aim of this study was to examine the effects of recombinant human Hsp70 (rhHsp70) when present alone or together with bacterial components lipopolysaccharide (LPS) and lipoteichoic acid (LTA) as well as cigarette smoke extract (CSE) on activation of mitogen-activated protein kinases (MAPKs) signalling pathways in bronchial-epithelial NCI-H292 cell line. Methods: NCI-H292 cells were treated either with rhHsp70 (0.3, 1.0 or 3.0 µg/mL), 0.1 µg/ml LPS, 1 µg/ml LTA and CSE (2.5% or 15%) alone, or with their combinations, for 30 min, 2 h, 8 h and 24 h. Expression and activation of MAPKs (ERK, JNK and p38) were determined by Western blotting method. Results: rhHsp70, LPS, LTA and their combinations caused activation of ERK, JNK and p38 MAPK, while rhHsp70 together with CSE caused activation of ERK and p38 MAPK in NCI-H292 cells, compared to the control cells. Extent of activation was dependant on concentration and time of treatment. Conclusions: eHsp70 as well as bacterial components (LPS and LTA) and CSE activate MAPK signalling pathways in NCI-H292 cells. Combined presence of eHsp70, LPS, LTA and/or CSE might lead to worsening of COPD patients’ condition.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti, Temeljne medicinske znanosti, Kliničke medicinske znanosti, Farmacija

POVEZANOST RADA