Naslov
Combined effect of extracellular Hsp70 and cigarette smoke extract on cytokine secretion from bronchial-epithelial NCI-H292 cell line

Autori
Grdić Rajković, Marija ; Hulina, Andrea ; Somborac Bačura, Anita ; Jelić, Dubravko ; Vukić Dugac, Andrea ; Rumora, Lada

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
ERS International Congress 2018 / - : European Respiratory Society, 2018, 3311-3311

Skup
28th International Congress of the European Respiratory Society (ERS 2018)

Mjesto i datum
Pariz, Francuska, 15.09.2018. - 19.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
chronic obstructive pulmonary disease ; extracellular Hsp70 ; cigarette smoke ; NCI-H292 cell line

Sažetak
Introduction: Cigarette smoke is one of the major risk factors for development of chronic obstructive pulmonary disease (COPD), which is characterized by chronic inflammation. Extracellular Hsp70 is increased in sera of COPD patients, and can act as damage-associated molecular pattern. Aims and objectives: We explored the pro-inflammatory effects of cigarette smoke extract (CSE) alone and in combination with recombinant human Hsp70 (rhHsp70) on bronchial-epithelial NCI-H292 cell line. Methods: NCI-H292 cells were treated with 2.5% or 15% CSE alone or in combination with 0.3, 1.0 or 3.0 µg/mL rhHsp70 for 24 h. IL-1α, IL-6 and IL-8 were determined in cell supernatants by ELISA method. Results: Compared to the non-treated cell, secretion of IL-1α, IL-6 and IL-8 was significantly increased after treatment with 2.5% CSE, but not in combination with rhHsp70. Combination of rhHsp70 and CSE resulted in different secretion of IL-1α, IL-6 and IL-8 compared to the cells treated with CSE alone, and theirinteractions were antagonistic. Concentrations of IL-1α, IL-6 and IL-8 were different after treatment of cells with 15% CSE or combination of CSE and rhHsp70 compared to the non-treated cells, but their concentrations did not differ after combined treatment compared to CSE alone.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti, Temeljne medicinske znanosti, Kliničke medicinske znanosti, Farmacija

POVEZANOST RADA