Naslov
Overexpression of YAP1 gene increases the resistance of yeast strains to inhibitors found in lignocellulosic hydrolysates

Autori
Štafa, Anamarija ; Djedović, Elvis ; Moguš, Leo ; Žunar, Bojan ; Svetec Miklenić, Marina ; Šantek, Božidar ; Svetec, Ivan Krešimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
4th Congress of Croatian Geneticists with International Participation Book of Abstracts / - Zagreb : Hrvatsko genetičko društvo, 2018, 66-66

ISBN
978-953-57128-1-7

Skup
4th Congress of Croatian Geneticists with international participation

Mjesto i datum
Krk, Hrvatska, 26.09.2018. - 29.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
gene targeting, targeted chromsome duplication, genome duplication

Sažetak
Due to the constant increase in energy consumption, the production of biofuels from renewable sources, such as lignocellulosic waste, has become more frequent in the last several decades. Lignocellulosic waste consists of cellulose, hemicellulose and lignin and the yeast S. cerevisiae is not able to ferment it directly. Therefore, lignocellulosic waste is usually hydrolysed to release compounds that can be used for fermentation. However, the pretreatment also generates compounds that act as growth and fermentation inhibitors such as acetic and levulinic acid and 2-furaldehyde. In this study, newly constructed intraspecies hybrid diploids, obtained by crossing natural S. cerevisiae isolates, showed higher resistance to acetic and levulinic acid. Selected strains were also transformed with replicative vectors overexpressing yeast genes (e.g. ATR1, CTA1, FLR1, YAP1, ZWF1) encoding proteins involved in a stress response and the overexpression of YAP1 gene further increased the resistance to inhibitors. Therefore, our results suggest that the construction of intraspecies hybrids coupled with genetic engineering is a promising approach for construction of new biotechnologically relevant yeast strains.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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