Naslov
Association of serum endothelial lipase with levels and functional characteristics of HDL particles and endothelial dysfunction in patients with metabolic syndrome

Autori
Klobučar, Iva ; Degoricija, Vesna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
PhD Day 2018 Abstract Book / - Zagreb : University of Zagreb, School of Medicine ; Medicinska naklada, 2018, 119-119

ISBN
978-953-176-848-1

Skup
Dan doktorata 2018 = PhD Day 2018

Mjesto i datum
Zagreb, Hrvatska, 25.05.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
endothelial lipase ; HDL ; endothelial dysfunction

Sažetak
Introduction: HDL particles induce NO synthesis in vascular endothelial cells, inhibit LDL particles oxidation and reduce adhesion molecules expression on endothelial cells, resulting in vasodilation and anti-inflammatory effect. Endothelial lipase is an enzyme which degrades HDL particles and its concentration is increased in metabolic syndrome. Endothelial dysfunction is a state of impaired vasodilation potential and presents the first and still reversible step to atherosclerosis. Hypothesis: Increased serum concentration of endothelial lipase in the patients with metabolic syndrome is associated with lower HDL particle levels, resulting in the development of endothelial dysfunction. Endothelial lipase concentration could be a measure of the occurrence and severity of early, subclinical stages of atherosclerosis. Aims: The aim of this study is to describe relation between the serum levels of endothelial lipase, serum levels of HDL particles and the stage of endothelial dysfunction of the brachial artery (established indicator of subclinical atherosclerosis), in healthy volunteers and patients with metabolic syndrome. Materials and methods: This observational, cross- sectional study in the University Hospital Centre Sisters of Charity, involves 130 individuals - 65 healthy volunteers and 65 patients with metabolic syndrome. Criteria for the diagnosis of metabolic syndrome are defined by A Joint Interim Statement of the International Diabetes Federation Task Force on Epidemiology and Prevention ; National Heart, Lung, and Blood Institute ; American Heart Association ; World Heart Federation ; International Atherosclerosis Society ; and International Association for the Study of Obesity from 2009. Exclusion criteria are any acute disease or chronic disease worsening within 6 months prior to the inclusion in the study, severe renal insufficiency and liver cirrhosis, malignant and autoimmune diseases. Patient history will be recorded and physical examination performed. A total of 24 ml of venous blood will be taken from each patient, centrifuged and serum stored at the temperature of -80°C until the laboratory analysis in the Institute of Molecular Biology and Biochemistry of the Medical University of Graz, Austria. Beside standard laboratory biochemistry tests, concentration of endothelial lipase and lipoprotein particles will be measured from the serum samples. Endothelial dysfunction will be evaluated using ultrasound measurement of brachial artery diameter changes after stimulation of endogenous NO production (FMD - flow-mediated dilation) and after application of nitroglycerin (NMD - nitroglycerin-mediated dilation). Brachial artery diameter changes will be measured automatically and continuously using software FloWave.US v. 0.2.0 (Coolbaugh CL, Vanderbilt University Institute of Imaging Science, Nashville, Tennessee, USA), in accordance with currently valid guidelines. All data will be manually imported in the electronic database in Microsoft Excel 2010 (Microsoft, Redmond, Washington, USA) and statistically analysed using IBM SPSS Statistics Version 25 (IBM Corporation, Armonk, New York, USA). Study is a part of scientific project Endothelial lipase, HDL and endothelial dysfunction, supported by Austrian Science Foundation (FWF ; grant P 27166-B23). Research is conducted in cooperation with the Institute of Molecular Biology and Biochemistry, Medical University of Graz, Austria (Agreement on cooperation between the University of Zagreb and Medical University of Graz, 21 February 2005. Principal investigators: Prof. Sa{; ; ; ; a Frank (Graz) and Prof. Vesna Degoricija). Expected scientific contribution: Expected scientific contribution of the study is discovering further information about endothelial lipase function and elucidating its role in the development of atherosclerosis in humans. Endothelial lipase serum concentration will be evaluated as a novel indicator of the occurrence and severity of subclinical atherosclerosis.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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