Naslov
Bacterial isolates from crayfish surface biofilm as potential source of anti-omycete compounds

Autori
Orlić, Karla ; Šver, Lidija ; Burić, Lucija ; Kazazić, Snježana ; Hrašćan, Reno ; Vladušić, Tomislav ; Bielen, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Programme and Abstracts of 6th Central European Symposium on Antimicrobials and Antimicrobial Resistance CESAR 2018 / Abram, Maja ; Bielen, Ana ; Kifer, Domagoj ; Maravić Vlahoviček, Gordana ; Šegvić Klarić, Maja - Zagreb : Hrvatsko mikrobiološko društvo, 2018, 104-104

ISBN
978-953-7778-16-3

Skup
6th Central European Symposium on Antimicrobials and Antimicrobial Resistance (CESAR 2018)

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 19.09.2018. - 22.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Aphanomyces astaci ; crayfish epibionts ; anti-oomycete activity

Sažetak
Oomycetes (water moulds) are a group of eukaryotic microorganisms that include many pathogens of terrestrial and aquatic species. Among freshwater oomycetes, microorganism Aphanomyces astaci (Schikora, 1906) is a causative agent of lethal disease crayfish plague and a reason for decline of native decapod crayfish populations all over Europe. During the infection, germinating A. astaci zoospores penetrate through the crayfish cuticle, followed by growth of hyphae through the host tissue. However, upon the arrival of zoospores on the crayfish cuticle, they encounter host epibiontic biofilm communities. While the protective role of commensal microbes has been shown in other models, the interaction of A. astaci and host biofilm is completely unexplored. Therefore, the aim of this work was to explore the potential of bacterial isolates originating from crayfish cuticle to inhibit A. astaci growth. We took swabs of cuticle biofilm from five specimens of narrow-clawed crayfish, Astacus leptodactylus Eschscholtz, 1823 and five specimens of signal crayfish, Pacifastacus leniusculus (Dana, 1852) and inoculated them on PG1 medium. Next, we have isolated morphologically different individual colonies from mixed cultures and taxonomically identified each isolate by MALDI–TOF mass spectrometry and 16S rRNA gene sequencing. Dominant bacterial genera among isolates were Pseudomonas and Acinetobacter. Furthermore, we tested the potential of isolates to inhibit the growth of A. astaci mycelium by plate inhibition assay. Out of 46 isolates from narrow-clayed crayfish, 13 exhibited great potential for A. astaci inhibition (28 %), while out of 44 isolates from signal crayfish, only 7 showed great inhibition potential (16 %). Most of the potent inhibitors belonged to genus Pseudomonas, for which inhibition of fish pathogenic oomycete from genus Saprolegnia was previously shown. In conclusion, we have characterized cultivable crayfish-associated biofilm communities and shown their potential as a source of anti- oomycete compounds.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

POVEZANOST RADA