Naslov
Antitumour activity of an aqueous garlic extract in vitro

Autori
Mišković Špoljarić, Katarina ; Jakovljević, Martina ; Jokić, Stela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts:17th Ružička days "Today Science-Tomorrow Industry" / Tomas, Srećko ; Ačkar, Đurđica - Osijek : Zagreb : Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku ; Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2018, 98-98

ISBN
978-953-7005-57-3

Skup
17. Ružičkini dani: Danas znanost-sutra industrija = 17th Ružička days: Today science-tomorrow industry

Mjesto i datum
Osijek, Hrvatska; Vukovar, Hrvatska, 19.09.2018. - 21.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
aqueous garlic extract ; cold plasma ; antitumor activity ; MTT test

Sažetak
Tumor is malignant disease characterized with uncontrolled cell growth and division along with contingency to form metastasis. In straggle to prevent development of malignant disease notable part belongs to food with significant bioactivity. Garlic (Allium sativum) is known as a foodstuff with prophylactic and therapeutic properties. It is biological activity arise from allin which disassemble to alicin and active sulphide components. Various procedures of aqueous extraction leads to extracts with different assembling of biologically active compounds. Evaluated extract was prepared by application of cold atmospheric plasma assisted extraction (CAPAE) while extract composition was determined by GC-MC method. Antitumor activity was evaluated by MTT test on four human cell lines (HeLa, CaCo-2, MCF-7, NCI-H358). Data were analyzed by Mann Whitney U test (p<0.05) in STATISTICA 11 software. Application of CAPAE give aqueous garlic extract with 17 different components. Among them, one was active sulphide component known as di-2-propenyl or disulphide. with 8, 061 minutes of a retention time. Antitumor activity of garlic extract was tested in concentration range from 2-5 mg/ml. The highest efficiency in suppression of tumor cell growth was determined at the concentration of 4 mg/mL which inhibited cell growth of CaCo-2 by 90.6%, HeLa by 84.1%, MCF 7 by 82.6% and NCI- H358 by 100%). CAPAE is a new approach in preparation of aqueous garlic extracts. Complex composition of different detected components probably act in synergy producing recorded inhibitory effect on tested cell lines.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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