Naslov
Challenges in appendicularian integrative taxonomy and barcoding

Autori
Garić, Rade ; Pfannkuchen, Martin ; Denes, Marcell ; Gissi, Carmela ; Flood, Per R. ; Batistić, Mirna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
ICES Annual Science Conference 2018 / - , 2018

Skup
International Conference on Environmental Systems focuses on the dissemination of technical and scientific information (ICES 2018)

Mjesto i datum
Hamburg, Njemačka, 24.09.2018. - 28.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Appendicularia ; integrative taxonomy ; barcoding

Sažetak
Appendicularians are a group of planktonic tunicates which usually comprise about 10% of total mesozooplankton. Despite their importance in marine plankton communities little has been done in their integrative taxonomy or barcoding. Appendicularians possess very high evolutionary rates which makes designing of universal barcoding primers a challenge. This problem is further aggravated with the discovery of poly-T insertions in mitochondrial genes of Oikopleura dioica which renders mitochondrial genes practically unsequenceable by common sequencing methods. Special challenges in sequencing appendicularian genes in combination with very few available taxonomic experts has lead to a situation where there are practically no appendicularian population genetic studies and very few available cox1 and 18S sequences. In order to gain insight in appendicularian genetic diversity and phylogenetic relationships, we produced a comprehensive set of appendicularian 18S sequences as well as ITS1-5.8S-ITS2-28S fragment. Based on obtained data we re-assess the status of currently established genera and validity of some appendicularian subspecies. We also discuss problems in appendicularian sampling and some potential candidates for barcoding genes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA