Naslov
Biochemical response of Arabidopsis thaliana transgenic plants with a disturbed function of enzyme auxin amidohydrolase after salt stress exposure

Autori
Vujčić Bok, Valerija ; Radić Brkanac, Sandra ; Pavlović, Iva ; Linić, Ida ; Salopek-Sondi, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts of the 13th Croatian biological congress / Kružić, Petar ; Caput Mihalić, Katarina ; Gottstein, Sanja ; Pavoković, Dubravko ; Kučinić, Mladen - Zagreb : Hrvatsko biološko društvo, 2018, 215-216

Skup
13. Hrvatski biološki kongres

Mjesto i datum
Poreč, Hrvatska, 19.09.2018. - 23.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Arabidopsis thaliana, transgenic plants, salt stress, biochemical parameters

Sažetak
Biochemical response of Arabidopsis thaliana mutants with impaired function of the enzyme auxin amidohydolase (single ilr1, iar3, and ill2 ; double iar3 ill2 and triple ilr1 iar3 ill2) and the corresponding wild type (wt) of the Wassilewskii ecotype to high salinity (100 mM NaCl) was evaluated after period of 7 days. Auxin amidohydrolases are enzymes that participate in homeostasis of plant hormone auxin by hydrolyzing auxin amide-conjugates and releasing active auxins. The contents of malondialdehyde, proline and ascorbate were determined as well as content of reactive oxygen species (superoxide radical SOˑ and hydrogen peroxide -H2O2) and glutathione using fluorescent probes dihydroethidium (DHE), dichlorodihydrofluorescein diacetate (DCFH-DA) and monochlorobimane (MCB). Salt stress induced significant decrease of ascorbate in ill2 and increase in iar3, iar3 ill2 and iar1 iar3 ill2. Accumulation of proline was significant not only in wt but also in all mutants exposed to salt stress. There was a statistically significant increase in the content of MDA (ilr1, iar3, iar3 ill2 and ilr1 iar3 ill2), glutathione (wt, ill2 and ilr1) and reactive oxygen species (SOˑ ; ilr1 and H2O2 ; ill2) upon salt stress. The highest content of ascorbate, proline and MDA after exposure to salt stress was measured in triple mutant. Based on the obtained results we can conclude that the triple mutant, which has the lowest ability to hydrolyze auxin conjugates, is the most sensitive to salt stress.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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