Naslov
Functional and molecular characterization of a peptide transporter in the rat PC12 neuroendocrine cell line

Autori
Hussain, Imran ; Žanić-Grubišić, Tihana ; Kudo, Yoshi ; Boyd, Carl Adam

Izvornik
FEBS Letters (0014-5793) 508 (2001), 3; 530-354

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
peptide transporter; PC12

Sažetak
We have studied functional properties of peptide transport in the pheochromocytoma neuroendocrine cell line from rat. The neutral peptide D-Phe-L-Ala (resistant to hydrolysis) is a good substrate for uptake into these cells. Transport is substantially inhibited by diethylpyrocarbonate pretreatment and is stimulated by external acidification. It is sodium-independent and, unexpectedly, insensitive to membrane potential. Peptide uptake is inhibited by a wide variety of other di- and tripeptides but not by amino acids. The neuropeptide kyotorphin (opioid dipeptide (L-Tyr-L-Arg)) inhibits uptake of labelled peptide and trans-stimulates efflux showing that it is a transported substrate. These findings are discussed in relation to the molecular basis and physiological role of this transport system.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA