Naslov
The Role of Apoptosis in Development of Chronic Tubulointerstitial Nephritis

Autori
Petrik, Jozsef ; Čepelak, Ivana ; Barišić, Karmela ; Pepeljnjak, Stjepan ; Ferenčić, Željko ; Žanić-Grubišić, Tihana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
3rd Croatian Congress of Pharmacology with International Participation : Abstract book ; u: Periodicum biologorum. Supplement / Vitale, Branko - , 2001, 102-102

Skup
Croatian Congress of Pharmacology with International Participation (3 ; 2001)

Mjesto i datum
Zagreb, Hrvatska, 18.09.2001. - 21.09.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
apoptosis; chronic tubulointerstitial nephritis

Sažetak
Ochratoxin A (OTA) is a widespread mycotoxin produced by several species of fungi, Aspergillus, Penicillium and Eurotium. OTA is the potent nephrotoxic, genotoxic and carcinogenic agent, and it affects normal blood coagulation and immune response. In vitro, OTA induced the apoptosis in different cell lines. It has been implicated as one of the etiologic agents involved in the development of Endemic nephropathy. OTA induces a tubular-interstitial nephropathy in humans and in animals. Toxic effects of OTA in kidneys are usually connected with primary lesions of proximal and distal tubules and involution of interstitium. It has been suggested that impairments in the kidney cells that have been observed after the exposure to low concentration of OTA are different to those seen with high OTA concentration. The observed differences are most probably due to changes in the complex regulatory processes. In this study we examined the effects of low doses of OTA on kidney. Wistar rats were treated with 120 mg OTA/kg body weight daily, during 10, 30 and 60 days (n=32). We detected the apoptosis in kidney tissue, and determined the renal status as well as OTA concentrations in serum, urine and kidney cortex. We used the TUNEL assay (Terminal deoxynucleotidyl transferase-mediated dUTP nick and labelling), DNA electrophoresis and morphological methods for detection of apoptosis. OTA treatment caused the increased number of apoptosis in the epithelial kidney cells. DNA analysis not showed characteristic fragmentation (DNA laddering). TUNEL assay and staining of kidney tissue with haematoxylin and eosin were visualized the apoptotic cells in situ. The number of apoptotic cells in 10, 30 and 60 days treated rats increased by 5, 6.4 and 12.7 fold, respectively, compared to the control cells. Toxin concentration in kidney was proportional to the time of exposure, and amount 547.2ng OTA/g, 752.5ng OTA/g and 930.3ng OTA/g kidney tissue after 10, 30 and 60 days, respectively. This study demonstrates that a combination of morphologic and biochemical markers can be used to distinguish predominant modes of cell death in OTA induced renal injury. The results of this work showed, that OTA, in relatively low concentration, is able to activate processes that stimulate apoptosis in renal tissue or in kidney cell lines. This study suggests that apoptosis stimulated by OTA may be involved in the development of chronic tubulointerstitial nephritis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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