Naslov
In the quest for new targets for pathogen eradication: the adenylosuccinate synthetase from the bacterium Helicobacter pylori

Autori
Bubić, Ante ; Mrnjavac, Natalia ; Stuparević, Igor ; Łyczek, Marta ; Wielgus-Kutrowska, Beata ; Bzowska, Agnieszka ; Luić, Marija ; Leščić Ašler, Ivana

Izvornik
Journal of Enzyme Inhibition and Medicinal Chemistry (1475-6366) 33 (2018), 1; 1405-1414

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Helicobacter pylori ; adenylosuccinate synthetase ; enzyme kinetics ; enzyme inhibition ; oligomeric state ; analytical ultracentrifugation

Sažetak
Adenylosuccinate synthetase (AdSS) is an enzyme at regulatory point of purine metabolism. In pathogenic organisms which utilise only the purine salvage pathway, AdSS asserts itself as a promising drug target. One of these organisms is Helicobacter pylori, a wide-spread human pathogen involved in the development of many diseases. The rate of H. pylori antibiotic resistance is on the increase, making the quest for new drugs against this pathogen more important than ever. In this context, we describe here the properties of H. pylori AdSS. This enzyme exists in a dimeric active form independently of the presence of its ligands. Its narrow stability range and pH-neutral optimal working conditions reflect the bacterium’s high level of adaptation to its living environment. Efficient inhibition of H. pylori AdSS with hadacidin and adenylosuccinate gives hope of finding novel drugs that aim at eradicating this dangerous pathogen.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Interdisciplinarne prirodne znanosti

POVEZANOST RADA