Naslov
Cell response to oxime treatment

Autori
Zandona, Antonio ; Katalinić, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Final Programme & Book of Abstracts, FEBS3+ coference From molecules to living systems, Siófok, Mađarska, 2018 / Szüts, Dávis ; Buday, László - Veszprém, 2018, 232-232

ISBN
978-615-5270-47-5

Skup
FEBS3+ conference "From molecules to living systems"

Mjesto i datum
Siófok, Mađarska, 02.09.2018. - 05.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
oximes ; cytotoxicity ; viability ; SH-SY5Y cells

Sažetak
Compounds known as oximes are used as antidotes in cases of organophosphorus compounds (OPCs) poisoning. OPCs are still used globally as pesticides or chemical weapons in attacks, as we have witnessed in recent years. The currently leading oxime antidote candidates are selected through in vivo testing, mostly without conducting any test on cell level to determine the adverse effects. We tested such candidates here in vitro on several cell lines to determine the effect on cell viability. As an additional indicator of cell status, change of ATP concentration and induction of reactive oxygen species were determined as well. The results were compared with the standard HI-6 oxime, which is used as medical treatment in OPC-recovery therapy. Our results indicate that several oximes induced cell death via apoptosis or other mechanisms, and were toxic to cells. Such results are another indication that it is necessary to carry out cell-based assays prior to the selection of leads and in vivo use. Acknowledgment: This work was supported in part by the Croatian Science Foundation under the project UIP-2017-05-7260.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA