Naslov
Clinically significant influence of haemolysis on 25 biochemistry parameters

Autori
Perović, Antonija ; Dolčić, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Biochemia Medica / - , 2018, 95-96

Skup
9. kongres hrvatskog društva za medicinsku biokemiju i laboratorijsku medicinu (HDMBLM)

Mjesto i datum
Zagreb, Hrvatska, 09.05.2018. - 12.05.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Hemolysis ; Interference ; Laboratory testing ; Preanalytical errors ; Reference change value

Sažetak
Introduction: Manufacturers’ recommendations on hemolysis influence on tests parameters are often scarce, non-harmonized and therefore should be evaluated prior to implementation in routine work. The aim of this study was to investigate influence of hemolysis on four different hemolysis levels for 25 biochemistry parameters and to evaluate the clinical significance of hemolysis in order to prevent unnecessary sample rejection. Materials and Methods: A total of 17 venous blood samples collected in gel-separator tubes were included in the study. The samples were immediately spiked with high concentrations of tested parameters and a 1 mL aliquot was taken for hemolysate preparation by rapid freeze-thaw method. The non- hemolyzed serum was divided into five aliquots. Four hemolysis levels were prepared by adding hemolyzed serum. Hemolysis index (HI) and hemoglobin concentration in prepared samples were classified as following: (+)=0.5-0.99 g/L, (++)=1- 1.99 g/L, (+++)=2-2.99 g/L, (++++)=3- 4.99 g/L. The following parameters were determined on Beckman Coulter AU680 analyzer: IH, potassium, sodium, chloride, calcium, magnesium, inorganic phosphates, iron, glucose, total and conjugated bilirubin, urea, creatinine, uric acid, alkaline phosphatase (ALP), gamma- glutamyltransferase (GGT), aspartate aminotransferase (AST), alanine aminotransferase (ALT) creatine kinase (CK), CK- MB, lactate dehydrogenase (LD), alpha- amylase, cholinesterase, C-reactive protein (CRP), total protein, albumin. The percentage differences between non- hemolytic and hemolytic serum judged against desirable bias (DSB) derived from biological variation and reference change values (RCV) calculated according to the following formula: RCV=21/2×Z×(CVA2+CVI2)1/2, where Z=1, 96 ; CVA=calculated analytical imprecision ; CVI= intraindividual biological variation. The difference higher than RCV was considered clinically significant. Results: The differences exceeded DSB for: potassium, total and conjugated bilirubin, AST, CK-MB, LD at IH= (+) ; sodium, magnesium, inorganic phosphates, GGT, total protein at IH=(++) ; calcium, CK, alpha-amylase, albumin at IH= (+++). The differences exceeded RCV for: LD at IH=(+) ; CK-MB at IH=(++) ; potassium, AST at IH=(+++). Conclusion: Hemolysis influence is clinically significant for LD at IH=(+), CK-MB at IH=(++), potassium, AST at IH=(+++).

Izvorni jezik
Engleski

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