Naslov
Quantitative microbial assessment for Escherichia coli after treatment by high voltage gas phase plasma

Autori
Stulić, Višnja ; Vukušić, Tomislava ; Režek Jambrak, Anet ; Bačun-Družina, Višnja ; Popović, Dean ; Mrvčić, Jasna ; Herceg, Zoran

Izvornik
Innovative food science & emerging technologies (1466-8564) 53 (2018); 26-35

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Escherichia coli ; plasma ; reduction ; cell recovery ; cellular leakage ; fluorescence microscopy

Sažetak
High voltage electrical discharge (gas and liquid) plasmas are new developing techniques used for food and water decontamination. This study investigates the influence of high voltage gas phase plasma treatment on several parameters: inactivation of Escherichia coli K12, recovery, proteomic analyses, cellular leakage and influence of pH and generated H2O2 species on inactivation of the E. coli cells. Samples were treated in a glass reactor with a point-to-plate electrode configuration. Electrical conductivity (100 and 800 μS/cm), polarity (+/−), time (5 and 10 min) and frequency (60, 90 and 120 Hz) were chosen in order to determine their influence on listed parameters. Statistical analyses were obtained to optimize conducted treatments. Treatment regime of 90 Hz, positive polarity, 100 μS/cm and 10 min resulted in the highest reduction (7.8 log10 CFU/mL) of E. coli. Recovery of cells after the treatment suggested oxidative stress overcome.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA