Naslov
Does Hansenula Polymorpha CBC 4732 Ferment Sucrose Under Oxygen-Limited Conditions? Comparison of Durham test and Chemostat Experiments

Autori
Kaliterna, Janko ; Mihaljević, Krešo ; Novak, Srđan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Biotechnology and Environment / Kniewald, Zlatko - Zagreb : Hrvatsko Društvo za Biotehnologiju, 2001

Skup
Biotechnology and Environment

Mjesto i datum
Zagreb, Hrvatska, 19.02.2001. - 22.02.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
H. polymorpha; fermentation ability; Durham test; chemostat

Sažetak
A facultatively fermentative yeast Hansenulta polymorpha CBS 4732 is referred in literature as a yeast that does not ferment disaccharides in oxygen-limited conditions. Results of Durham test confirm literature data showing that H. polymorpha does not ferment sucrose but ferment glucose as control sugar intensively producing carbon dioxide bubbles after three day cultivation. In contrary, growth of H. Polymorpha in chemostat under oxygen limited conditions resulted with significant ethanol production. An active fermentative metabolism on sucrose in such controlled conditions is in contradiction with results of Durham test and literature data. In this work reasons for unreliability of Durham test are discussed and alternative methods for estimation of fermentative ability of yeasts are recommended.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija

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